Background: Enhancing nurses' job attitude in the organization could be achieved through Organizational Citizenship Behavior, better the performance of employees, job satisfaction and organizational productivity .The study aimed to: assess the relation between job attitude and organizational citizenship behavior among staff nurses in Health Insurance Hospital. Research design: A descriptive correlational research design. Sample: consisted of all available staff nurses (196 nurses) working in Health Insurance Hospital during data collection. Setting: conducted at Health Insurance Hospital, Minia City. Tools of data collection: two tools were used, tool І: job attitude scale  and tool II: organizational citizenship scale  were used. Results: the findings of the current study reveals that (68.4%) of staff nurses have positive job attitude while (31.6%)of them have negative job attitudes. Also (60.7%) of staff nurses have moderate level, followed by (39.3%) from them have high level of the total organizational citizenship behavior.  Conclusion: there were a positive correlation between job attitude and organizational citizenship behavior. Recommendations: Conduct an educational program for all levels of nurses about the organizational citizenship behavior to spread this behavior among them.