The study investigated the effects of acrylamide (ACR) on the liver and kidneys in rats and the potential protective and therapeutic properties of Moringa oleifera (M. oleifera) leaf nanoparticles, using UV-visible spectroscopy to create and characterize silver nanoparticles. 20 adult male rats were randomly divided into four groups: Control group (CT); Acrylamide group (ACR): rats received 50 mg/kg b.wt. in drinking water for 3 weeks; Protection group (Mo-NPs /ACR): rats received 50 mg/kg b.wt. of M. oleifera nanoparticles (Mo-NPs) daily for 3 weeks and were given 50 mg/kg b.wt. of acrylamide (ACR) daily for 3 weeks; Treatment group (ACR/Mo-NPs): rats were given 50 mg/kg b.wt. of acrylamide (ACR) for 3 weeks, followed by M. oleifera nanoparticles (Mo-NPs) for 3 weeks. Blood and tissue samples were obtained for the physiological and histological investigations, and a comet assay was used to determine the amount of DNA damage. Administration of ACR increased MDA, creatinine, urea, ALT, and AST activities while decreasing SOD enzyme activity. M. oleifera nanoparticle treatment raised SOD enzyme activity and decreased the damaging effects of ACR on these levels. Rats with ACR injuries treated with M. oleifera nanoparticles had improved histological abnormalities in their liver and kidneys. Greater DNA damage was seen in the liver cells of the ACR group; however, M. oleifera nanoparticles may have repaired this damage in other groups. The study concluded that M. oleifera nanoparticles provide enhanced protection against ACR's effects on liver and kidney function in rats, potentially due to its diverse phytochemicals.