The effect of Artemisia absinthium and Cyproheptadine on the insulin levels of rats fed on a diet deficient in zinc was examined in the current study.Thirty-two mature male rats were divided randomly into four groups each of eight animals. Control Group (G1), Zinc Deficiency Group (ZDD) (G2), Zinc Plus Cyproheptadine Group (G3), and Zinc Plus Artemisia absinthium Group (G4). Zinc, Cyproheptadine and Artemisia absinthium were taken orally (containing zinc not more than 1mg/kg diet, 720 µg/kg B.W p.o, 100 mg/kg BWBW) for 30 days. Insulin level and histopathological study were done. The data showed that feeding rats on a zinc deficient-diet (ZDD) resulted in decrease of insulin level compared to the control group, On the other hand, the Cyproheptadine (CH) and Artemisia absinthium (ART) treatment groups exhibited improvement of insulin level compared to zinc deficiency diet feeding group. In addition, zinc deficient-diet (ZDD) showed mild fatty liver with hepatic steatosis, Macrovesicular Steatosis. After treatment with cyper and Art, liver of both groups are tissue improved significantly.treatment with artemisia absinthium (ART) and cyproheptadine (CH) of rats feed on a diet deficient in zinc, increase insulin levels and improve liver tissue histology.