There is a persistent need for new chemotherapeutic drugs, indicating a vital requirement for innovative techniques. The purpose of this study was to assess the protective capabilities of 4-((quinolin-2-yl) methyleneamino)-1,2-dihydro-2,3-dimethyl-1-phenylpyrazol-5-one (QMP) against the alterations in oxidative stress, hematological, and TNF-α generated by Ehrlich ascites carcinoma (EAC) cells in mice. The study involved the division of 100 adult male Swiss albino mice into 5 groups, with each group consisting of an equal number of mice. The groups were labeled as follows: G1 (negative control), G2 (1% DMSO), G3 (QMP), G4 (EAC), and G5 (EAC +QMP). The anticancer efficacy of (QMP) was determined by assessing antioxidant, hematological parameters, and the level of TNF-α. EAC group had significant elevations in hematological parameters, NO, MDA, and TNF-α levels. In contrast, the (EAC+ QMP) group had decreased levels of hematological parameters, NO, MDA, and TNF-α. The findings of our study suggest that (QMP) possesses anti-inflammatory and antioxidant capabilities, which have the ability to reduce oxidative stress in EAC cells by enhancing the system of antioxidant defense.