Explorations into the potential application of Fe-O/Ti-O composite as antibacterial agents have been of interest due to their enhanced antibacterial activity. Fe-O/Ti-O composite was successfully synthesized using ilmenite ore as the initial titanium source. The rocks were cut and then the non-oxidized inner part was ground to -200 mesh size by using a pulverizer. Also, heat-treated obtained powder at 300 and 600°C for 2 h were prepared. This synthesis method was cost-effective and straightforward due to the absence of the traditional gravity, magnetic, electrostatic separation, ball milling, and heat up processes. The ilmenite and heat-treated powder were characterized by UV/Vis spectrophotometer, Energy-dispersive X-ray spectrometry (EDX) and Fourier transform IR spectroscopy. The antibacterial action of the prepared powders was investigated against Gram-positive bacterium; Bacillus cereus, and Gram-negative bacterium; Escherichia coli. Our results showed that the antibacterial activity of ilmenite decreased by increasing heat treatment temperatures. Also, the powders had strong antibacterial potential against Gram-positive than Gram-negative bacteria.