300695

Cellulolytic activities of thermophilic Thermoascus aurantiacus MW559792 and Mycothermus thermophilus MZ723073.

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Microbiology and its applications

Abstract

Seven thermophilic species were isolated from various thermogenic habitats on modified Czapek's Dox agar medium with CMC replacing glucose. The isolated fungi were identified based on their morphological characters and sequence homology of their rRNA gene sequences. Congo red test was used to display their cellulolytic activity. The cellulolytic activity (FPase, CMCase and β-glucosidase) of five selected species were performed on solid state fermentation (SSF). Thermoascus aurantiacus MW559792 and Mycothermus thermophilus MZ723073 were selected for optimization experiment based on their maximum productivity of cellulases. Culture conditions were optimized under SSF experiment. Of the five lignocellulosic wastes used as carbon sources, rice straw was the most favorable for maximum production of FPase (1.78, 1.45 U/g), CMCase (6.18, 4.09 U/g) and β-glucosidase (31.93, 16.23 U/g) from T. aurantiacus and My. thermopilus respectively. The suitability of the lignocellulosic substrates was in the following order: rice straw> maize straw > wheat straw>corn cob> rice hulls. Peptone was the most suitable nitrogen source for maximum FPase (1.88, 1.46 U/g), CMCase (7.02, 4.89 U/g) and β-glucosidase (28.60, 19.91 U/g) activities by T. aurantiacus and My. thermophilus respectively. Nitrogen sources availability was in the following order: Peptone > Yeast Extract>(NH₄) ₂SO₄> Beef Extract> NH4NO3> NaNO3> KNO3. The maximum cellulolytic activities of T. aurantiacus and My. thermophilus were also achieved at pH 5, 50°C and 45°C and 4 to 6 days of incubation respectively. On that bases T. aurantiacus MW559792 and My. thermophilus MZ723073 were suggested to use industrially for bioconversion of lignocellulosic wastes at saccharification stage.

DOI

10.21608/sjdfs.2023.195180.1088

Keywords

Thermophilic fungi, Cellulases, FPase, CMCase, β -glucosidase

Authors

First Name

EL-Sayed

Last Name

EL-Morsy

MiddleName

M. M.

Affiliation

Botany and Microbiology , Fcaculty of sciences Damietta Univ.

Email

el_morsy@du.edu.eg

City

-

Orcid

-

First Name

Mohammad

Last Name

. El-Metwally

MiddleName

M

Affiliation

botany Dep., Fac. sciences Damnhour Univ.

Email

mmmyco@gmail.com

City

Mansoura

Orcid

-

First Name

Marwa

Last Name

Mohesien

MiddleName

T.

Affiliation

Botany and Microbiology , Fcaculty of sciences Damietta Univ.

Email

marwabotany@yahoo.com

City

New Damiatta

Orcid

-

First Name

sally

Last Name

Shabara

MiddleName

A.

Affiliation

Botany and Microbiology , Fcaculty of sciences Damietta Univ.

Email

sally572015@gmail.com

City

New Damiatta

Orcid

-

Volume

13

Article Issue

1

Related Issue

42094

Issue Date

2023-06-01

Receive Date

2023-03-12

Publish Date

2023-06-01

Page Start

102

Page End

113

Print ISSN

2314-8594

Online ISSN

2314-8616

Link

https://sjdfs.journals.ekb.eg/article_300695.html

Detail API

https://sjdfs.journals.ekb.eg/service?article_code=300695

Order

300,695

Type

Original articles

Type Code

2,045

Publication Type

Journal

Publication Title

Scientific Journal for Damietta Faculty of Science

Publication Link

https://sjdfs.journals.ekb.eg/

MainTitle

Cellulolytic activities of thermophilic Thermoascus aurantiacus MW559792 and Mycothermus thermophilus MZ723073.

Details

Type

Article

Created At

28 Dec 2024