The present study aimed to indicate the harmful effects of Acrylamide (ACR) on blood cell of rats and the protective role of silver nanoparticles (Ag-NPs) derived from Moringa oleifera (M.oleifera) leaves against this effects. Ag-NPs were produced and subsequently examined using Transmission Electronic Microscope and Dynamic Light scattering measurements. Twenty adult male albino rats were randomly divided into four groups (five per each):Control group; ACR group (50 mg/kg b.wt, in drinking water);MO-NPsACRS group (50 mg/kg b.wt, orally at the same time of each treatment) and Protective group, (MO-NPs/ACR group),MO-NPs for 3 weeks (50 mg/kg b.wt,orally) then ACR for 3 weeks (50 mg/kg b.wt). At the end of the experimental period, blood samples were collected in clean tubes containing anticoagulant for blood cells count.

Red blood cells counts (RBCs), hemoglobin levels (HB), the percentage of hematocrit value (HCT), Mean Corpuscular Volume (MCV), and Lymphocytes (LYM) were significantly decreased after administration of ACR. On the other hand, white blood cells counts (WBCs), Platelets (PLTs), MID cells and Granulocytes (GRAN) were significantly increased when compared with control group. Treatment with the nano-extract of M.oleifera significantly reduces the toxic effect of ACR on the counts of WBCs, PLTs, MID cells and GRAN cells. Furthermore, a significant increase and influence on the hematological counts of RBCs, LYM cells, level of HB, percentage of HCT and MCV was observed after administration of nano-extract of M.oleifera (p< 0.05).These results indicate that nano-extract of M.oleifera leaves enhances the protection against the hematological toxicity of ACR in male rats.