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399940

Study of Virulence Genes, Biofilm Formation and Antifungal Susceptibility of Medical Device Associated Candida Infection in ICU Hospitalized Patients

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Candida species are increasingly reported as a cause of hospital acquired infection. Biofilms play a role in Candida adherence to medical devices. Candida within biofilms is less susceptible to antifungal agents. HWP1, ALS1, ALS3, SAP1, PLB1 and INT1S are biofilm regulators genes. Objectives: the aim of the present work is to study medical device associated candida infection in ICU patients regarding virulence genes, biofilm formation and antifungal susceptibility. Methodology: Fifty candida isolates were identified by chromogenic media and VITEK 2 compact system and subjected to antifungal susceptibility test by modified disc diffusion method and VITEK -2. Biofilm study was carried out by microtiter plate method (MTP). Molecular study for virulence genes was done by conventional PCR. Results: Non albicans candida (NAC) were isolated at higher rate (64%) than candida albicans (36%). C. tropicalis was the commonest among non albicans. There was high agreement between Chromogenic media and VITEK -2 regarding identification of C.albicans and C.tropicalis. Kappa agreement was 0.87, 0.95 respectively. The resistance rates for candida isolates were as the followings: Fluconazole (28%), Amphotericin B (26%), Caspofungin (18%), Micafungin (16%), Voriconazole (14%) and (8%) for Flucytocine. HWP1, ALS1, ALS3 genes correlated with biofilm formation (p -values: 0.021, 0.001 and 0.014) respectively. Conclusion: Both NAC and Candida albicans are involved in device associated infection in ICU with higher resistance rate for Fluconazole and Amphotericin B. Drug resistance increases in isolates with biofilm forming capacity. Moreover, there was association between Virulence genes (HWP1, ALS 1 and ALS 3) and biofilm formation.

DOI

10.21608/ejmm.2024.344155.1403

Keywords

antifungal resistance, Biofilm, Candida, Device associated infection, Virulence genes

Authors

First Name

Reem

Last Name

Elkholy

MiddleName

M.

Affiliation

Department of Clinical Pathology Faculty of Medicine -Menoufia University

Email

reemmohsen97@yahoo.com

City

Menoufia

Orcid

-

First Name

Amira

Last Name

El-hendy

MiddleName

A.

Affiliation

Department of Clinical Pathology Faculty of Medicine -Menoufia University

Email

amiraalkilani88@gmail.com

City

-

Orcid

-

First Name

Hagar

Last Name

Nassar

MiddleName

S.M.

Affiliation

Department of Clinical Pathology Faculty of Medicine -Menoufia University

Email

hagarsaid76@gmail.com

City

-

Orcid

-

First Name

Eman

Last Name

Zaher

MiddleName

M.

Affiliation

Department of Clinical Pathology Faculty of Medicine -Menoufia University

Email

zahereman2011@yahoo.com

City

Shibin El kom

Orcid

0000-0001-6186-2710

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-14

Publish Date

2025-04-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_399940.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=399940

Order

399,940

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Study of Virulence Genes, Biofilm Formation and Antifungal Susceptibility of Medical Device Associated Candida Infection in ICU Hospitalized Patients

Details

Type

Article

Created At

28 Dec 2024