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398894

LncRNA HOTTIP and HOTTIP SNP rs1859168 in Colorectal Cancer

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Long non-coding RNA HOXA transcript at the distal tip (lncRNA HOTTIP) has been suggested as a tumor promoter in colorectal cancer (CRC). The emergence and spread of CRC have been associated with aberrant lncRNA expression. Single nucleotide polymorphisms (SNPs) are proven to be linked to cancer susceptibility and may influence the expression and function of lncRNAs. Objectives: This study aimed to evaluate the diagnostic and prognostic roles of lncRNA HOTTIP in CRC patients and to investigate the risk association between CRC susceptibility and HOTTIP SNP rs1859168. Methodology: This study included 30 CRC patients and 30 healthy controls. Gene expression of lncRNA HOTTIP in peripheral blood mononuclear cells (PBMCs) and tissue using real-time PCR (RT-PCR) was done. SNP genotyping of HOTTIP SNP rs1859168 was also done. Results: PBMCs and tissue expression levels of HOTTIP were upregulated in CRC cases with higher tissue expression than that of PBMCs in CRC cases. The frequency of the homozygous CC and the C allele of HOTTIP SNP rs1859168 was increased in CRC cases. Conclusion: The tissue and PBMCs expression levels of HOTTIP were able to diagnose CRC and differentiate between grade II and III CRC. Regarding HOTTIP SNP rs1859168, the homozygous CC and the C allele were associated with an increased risk of developing CRC.

DOI

10.21608/ejmm.2024.341714.1394

Keywords

HOTTIP, HOTTIP SNP rs1859168, CRC

Authors

First Name

Esraa

Last Name

Abd El-Ghani

MiddleName

H.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

dresraahassan91@aun.edu.eg

City

Assiut-Egypt

Orcid

-

First Name

Mohamed

Last Name

Elfeky

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

mohelfeky@hotmail.com

City

Assiut-Egypt

Orcid

-

First Name

Salwa

Last Name

Seif Eldin

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

salwaseifeldein@aun.edu.eg

City

Assiut-Egypt

Orcid

https://orcid.org/00

First Name

Mohamed

Last Name

Salem

MiddleName

A.

Affiliation

Department of Surgical Oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

abouelmagdabdallah@gmail.com

City

Assiut-Egypt

Orcid

-

First Name

Aliaa

Last Name

Ghandour

MiddleName

M.A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

aliaaghandour@yahoo.com

City

Assiut-Egypt

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-10

Publish Date

2025-04-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_398894.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=398894

Order

398,894

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

LncRNA HOTTIP and HOTTIP SNP rs1859168 in Colorectal Cancer

Details

Type

Article

Created At

28 Dec 2024