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Aberrant Expression of Circulating MicroRNA-373 and MicroRNA-660 as Biomarkers for Diagnosis of Breast Cancer in Egyptian Females

Article

Last updated: 09 Apr 2025

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Subjects

-

Tags

Diagnostics

Abstract

Background: Breast cancer (BC) is a leading cause of cancer-related mortality in women worldwide. Early detection significantly improves outcomes, yet current diagnostic methods, such as mammography and biopsy, have limitations, particularly in detecting early-stage disease. Circulating microRNAs (miRNAs) have emerged as promising non-invasive biomarkers for cancer detection. Objective: This study aims to evaluate the expression of serum miR-373 and miR-660-5p in breast cancer patients and their association with clinicopathological parameters. Methodology: This is a case-control study was conducted on 25 breast cancer patients and 25 age-matched healthy controls. Serum miRNA-373 and miRNA-660-5p levels were measured using quantitative real-time polymerase chain reaction (qPCR). Clinical and pathological data, including tumor stage, histological type, and molecular markers, were collected. Statistical analysis was performed to assess the differences in miRNA expression and their correlations with clinicopathological parameters. Results: Breast cancer patients exhibited significantly higher serum levels of miR-373 and miR-660-5p compared to controls (p < 0.001 and p = 0.030, respectively). MiR-373 expression was significantly associated with advanced tumor stages and nodal involvement (p = 0.008). However, miR-660-5p showed no significant correlation with clinicopathological features. The combined use of miR-373 and miR-660-5p improved diagnostic accuracy, with an area under the curve (AUC) of 0.859. Conclusion: Serum miR-373 and miR-660-5p are potential non-invasive biomarkers for breast cancer detection. MiR-373, in particular, shows promise in identifying advanced disease stages. Combining these miRNAs enhances diagnostic accuracy, offering a potential tool for early breast cancer diagnosis.

DOI

10.21608/ejmm.2024.338690.1380

Keywords

Breast cancer (BC), miRNA, circulating biomarkers, non-invasive diagnosis, qPCR

Authors

First Name

Hosam

Last Name

Anas

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

hosamanas@aun.edu.eg

City

Assiut

Orcid

-

First Name

Mona

Last Name

Hassan

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

monaaminhassan3@gmail.com

City

Assiut

Orcid

-

First Name

Mohamed

Last Name

El-Mokhtar

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

elmokhtarma@aun.edu.eg

City

Assuit

Orcid

-

First Name

Hanan

Last Name

Eltybe

MiddleName

A.

Affiliation

Department of Medical Oncology and Hematological Malignancies, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

hananahmed@aun.edu.eg

City

Assiut

Orcid

-

First Name

Hebatallah

Last Name

Hassan

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

heba.ismailhassan@gmail.com

City

Assiut

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-11-23

Publish Date

2025-04-01

Page Start

17

Page End

25

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 17-25.pdf

PDF . 492.0kB

Link

https://ejmm.journals.ekb.eg/article_395436.html

Detail API

http://journals.ekb.eg?_action=service&article_code=395436

Order

3

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Aberrant Expression of Circulating MicroRNA-373 and MicroRNA-660 as Biomarkers for Diagnosis of Breast Cancer in Egyptian Females

Details

Type

Article

Created At

28 Dec 2024