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392376

Antimicrobial and Cytotoxic Activities of Nigrospora oryzae Endophytic Fungi Isolated from Moringa oleifera

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Endophytic fungi are known to produce bioactive compounds with potential therapeutic applications. Objective: This study investigated the antimicrobial and cytotoxic activities of Nigrospora oryzae, an endophytic fungus, isolated from Moringa oleifera. Methodology: Nigrospora oryzae was cultured, and its secondary metabolites were extracted and screened for antimicrobial efficacy against a range of pathogenic microorganisms, including Bacillus subtilis ATCC 6051, Escherichia coli ATCC 8739, Klebsiella pneumoniae NCTC 13465, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. Results: The results demonstrated that extracts from Nigrospora oryzae exhibited high antimicrobial activity against Staphylococcus aureus and Candida albicans compared to other microorganisms. The cytotoxicity assays revealed a dose-dependent inhibition of cell proliferation in MDA-MB-231 cell lines, suggesting potential anticancer properties. IC50 values indicating moderate cytotoxicity for DCM extract (44.66 µg/ml) and superior efficacy for Doxorubicin (40.79 µg/m).  In silico docking validation of AutoDock Vina showed reliable RMSD values for DNA Gyrase B, DNA Topoisomerase 4, and PARP3, supporting the in vitro results. Among the identified compounds, Aurofusarin demonstrated the most promising inhibitory potential, with high binding affinities across all tested proteins (-11.44 to DNA Gyrase B, -8.40 to DNA Topoisomerase 4, and -11.55 to PARP3). While Mellein and Nigrospin showed lower activity, Aurofusarin's strong binding suggests significant pharmacological potential. Conclusion: This study highlights the antimicrobial and anticancer potential of Nigrospora oryzae extracts, particularly Aurofusarin, as a less toxic alternative to chemotherapy drugs like Doxorubicin. Further research should focus on its mechanisms of action and potential use in oncological therapies.

DOI

10.21608/ejmm.2024.334620.1368

Keywords

Endophytic fungi, Molecular identification, cytotoxicity assay, antimicrobial activity, Pharmacological applications

Authors

First Name

Aml

Last Name

Ghanem

MiddleName

-

Affiliation

School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt; Institute of Biotechnology for Postgraduate Studies and Research, Suez Canal University, Ismailia 41522, Egypt

Email

aml.ahmed.ghanem@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Al‐Karmalawy

MiddleName

A.

Affiliation

Department of Pharmaceutical Chemistry, College of Pharmacy, The University of Mashreq, Baghdad 10023, Iraq; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34518, Egypt

Email

akarmalawy@horus.edu.eg

City

-

Orcid

-

First Name

Noha

Last Name

Morsy

MiddleName

E.

Affiliation

Department of Food Science & Technology (Home Economics Branch), Faculty of Agriculture, Suez Canal University, Ismailia, 41522, Egypt

Email

noha.morsy2@gmail.com

City

-

Orcid

-

First Name

Mahmoud

Last Name

Elsabahy

MiddleName

-

Affiliation

Badr University in Cairo Research Center, Badr University in Cairo, Badr City, Cairo 11829, Egypt

Email

mahmoud.elsabahy@buc.edu.eg

City

-

Orcid

-

First Name

Ahmed

Last Name

Rayan

MiddleName

M.

Affiliation

Institute of Biotechnology for Postgraduate Studies and Research, Suez Canal University, Ismailia 41522, Egypt; Department of Food Technology, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt

Email

ahmed_rayan@agr.suez.edu.eg

City

-

Orcid

0000-0003-3544-4675

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-11-08

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_392376.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=392376

Order

392,376

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Antimicrobial and Cytotoxic Activities of Nigrospora oryzae Endophytic Fungi Isolated from Moringa oleifera

Details

Type

Article

Created At

28 Dec 2024