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392112

Diagnostic Potential of Serum TNFα and COX2 in ASD Disorder

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairment in social interactions, language delay and repetitive behavior of interest.  Inflammation plays a key role in ASD, with pro-inflammatory cytokines such as interleukin TNFa implicated in disease pathogenesis. This cytokines are produced by activated microglia and contribute to neuroinflammation, amyloid-beta plaque formation, and tau hyperphosphorylation. The diagnostic potential of TNFa in ASD remains underexplored. A number of neurological and mental problems have been associated with elevated COX-2 expression, and the enzyme is well-known to play an important role in inflammatory processes. Objectives: This study aims to compare serum levels of TNFa and COX2 in ASD  severity cases and healthy controls and investigates the diagnostic potential of these biomarkers using one way ANOVA. Methodology: The study enrolled 100 participants: 50 healthy controls and 50 ASD patients diagnosed according to CARS scal criteria. Serum TNFa and COX2 levels were measured using ELISA. Binary logistic regression, were employed to evaluate the diagnostic potential of these biomarkers. Results: ASD patients showed significantly (p-value <0.0001) elevated levels of TNFa, and COX2 compared to healthy controls. One way ANOVA analysis identified TNFa as a significant predictor of ASD with an TNFa levels in serum of the ASD group with its three stratified categories: mild, moderate, and severe. In mild cases the TNFa level 0.4740±0.072, the moderate cases 0.584±0.105 and for sever cases was 1.15186±0.498. Conclusions: The study concluded that elevated TNFα levels are strongly associated with ASD severity could serve as a promising diagnostic biomarker. Cox2, while elevated, did not show comparable diagnostic value.

DOI

10.21608/ejmm.2024.317029.1323

Keywords

Autism Sectrum Disorder, Inflammation, tumor necrosis factor alpha, Cyclooxygenase2

Authors

First Name

Fatima

Last Name

Almashhady

MiddleName

R.

Affiliation

Veterinary Microbiology Department Faculty of Veterinary Medicine, University of Kufa, Iraq; Branch of Internal Medicine, Faculty of Medicine, University of Kufa, Iraq

Email

fatimaradawi22@yahoo.com

City

Najaf

Orcid

-

First Name

Sauad

Last Name

Kadium

MiddleName

W.

Affiliation

Branch of Internal Medicine, Faculty of Medicine, University of Kufa, Iraq

Email

swkadium22@yahoo.com

City

Najaf

Orcid

-

First Name

Arafat

Last Name

Al-Dujaili

MiddleName

H.

Affiliation

Faculty of Science, Department of Biology, University of Kufa, Iraq

Email

arafathussein2@gmai.com

City

Najaf

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-08-31

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_392112.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=392112

Order

392,112

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Diagnostic Potential of Serum TNFα and COX2 in ASD Disorder

Details

Type

Article

Created At

28 Dec 2024