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391511

Development of Rapid Home-Made Immunochromatographic Test for Diagnosis of Cryptosporidium Parvum

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: Immuno-chromatographic Test (ICT) devices have emerged as valuable tools for diagnosing parasitic infections. These tests are cost-effective, user-friendly, and require minimal training, making them ideal for use in settings with limited resources. Additionally, ICT offers rapid results with high accuracy, which makes it an attractive alternative to more complex diagnostic methods. Objective: This study aimed to develop a homemade immune-chromatographic kit utilizing nanotechnology for detecting specific antigens of Cryptosporidium parvum in human stool samples. Methodology: A comparative analysis was conducted between two diagnostic methods (sandwich ELISA and ICT) using 56 human stool samples confirmed to be infected with Cryptosporidium by Ziehl–Neelsen staining. The study also included 30 stool samples infected with other parasites and 20 healthy stool samples as negative controls. Silica nanoparticles were conjugated with anti-Cryptosporidium antibodies for enhanced sensitivity in both assays. Results: For sandwich ELISA, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 92.9%, 91%, 91.8%, and 92.6%, respectively, for the Cryptosporidium-infected group (52 out of 56 positive). Five healthy controls and other non-Cryptosporidium parasitic infections were falsely positive. In contrast, the homemade ICT detected 54 out of 56 Cryptosporidium-infected samples as positive, with two false positives from the control group. Conclusion: The ICT demonstrated superior sensitivity and specificity compared to sandwich ELISA, making it a more reliable, cost-effective, and accurate tool for diagnosing Cryptosporidium infections, especially in resource-limited settings.

DOI

10.21608/ejmm.2024.332062.1365

Keywords

Cryptosporidiosis -, diagnostic test-, Silver nanoparticles -Sandwich ELISA-, Immunochromatography Test (ICT)

Authors

First Name

Mohamed

Last Name

Hendawy

MiddleName

A.

Affiliation

Department of Parasitology, Theodor Belharz Research Institute, Giza, Egypt

Email

magiclab2000@yahoo.com

City

Giza

Orcid

-

First Name

Ibrahim

Last Name

Aly

MiddleName

R.

Affiliation

Department of Parasitology, Theodor Belharz Research Institute, Giza, Egypt

Email

ibrahimshalash@yahoo.com

City

Giza

Orcid

-

First Name

Rabab

Last Name

Zalat

MiddleName

S.

Affiliation

Department of Parasitology, Theodor Belharz Research Institute, Giza, Egypt

Email

rababzalat@yahoo.com

City

Giza

Orcid

-

First Name

Shaimaa

Last Name

Mohamed

MiddleName

S.

Affiliation

Department of Parasitology, Theodor Belharz Research Institute, Giza, Egypt

Email

dr.shaimaashaker@gmail.com

City

Hay alashgar - 6th of October

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-10-29

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_391511.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=391511

Order

391,511

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Development of Rapid Home-Made Immunochromatographic Test for Diagnosis of Cryptosporidium Parvum

Details

Type

Article

Created At

28 Dec 2024