Beta
ManageSearchChatDiscover
390574

MicroRNA 29 gene Expression and Progesterone Receptor Values in Iraqi Women with Breast Cancer

Article

Last updated: 07 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Epidemiology

Abstract

Background: Globally as well as in Iraq, Breast cancer (BC) is the most common malignant tumor among malignancies that affect women and one of the most deadly illnesses that affect women. MicroRNAs (miRNAs) are 18–25-nucleotides regulatory non coding short RNAs that post-transcriptionally control the expression of genes to perform a wide range of essential functions in various biological processes. Objectives: The study aimed to evaluate the miR-29a expression level and association with breast cancer as well as to find out the relationship between miR29 and Progesterone receptor. Methodology: There were 74 of blood samples (54 breast cancer patients and 20 healthy control group).RNA extracted from patients and controls to synthesize complementary DNA(cDNA).Gene expression of miR29a was carried out using the quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: The results revealed that miR-29a gene expression in patients with breast cancer was (1.276851852), while in healthy people, the 2^-(∆∆Ct) was equal to zero, with a standard error of (0.281) and a dispersion deviation of (2.069) in comparison with control group. It was noted that women with breast cancer had an increased concentration of progesterone receptors, where the average concentration for healthy people was 0.348. Conclusion: From these findings we concluded that miR29 can serve as biomarker, diagnostic or predictive tool upon diagnosing breast cancer in the medical laboratories and also for treatment development. There was a significant association between mir29a and PR levels in this study.

DOI

10.21608/ejmm.2024.319913.1337

Keywords

Breast cancer(BC), Micro RNA, miRNA-29a, Progesterone receptor

Authors

First Name

Dhaneen

Last Name

Mahdi

MiddleName

-

Affiliation

Faculty of Science, Biology Department, Kufa University, Iraq

Email

dhanenm.alabudi@uokufa.edu.iq

City

najaf

Orcid

-

First Name

Mohammad

Last Name

Alzeyadi

MiddleName

-

Affiliation

Faculty of Science, Biology Department, Kufa University, Iraq

Email

mohamed.mhawish@uokufa.edu.iq

City

najaf

Orcid

-

First Name

Alaauldeen

Last Name

AL-Sallami

MiddleName

S.M.

Affiliation

Faculty of Science, Biology Department, Kufa University, Iraq

Email

aladdinalsallam@yahoo.com

City

najaf

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-10-09

Publish Date

2025-01-01

Page Start

149

Page End

155

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 1_Pages 149-155.pdf

PDF . 431.4kB

Link

https://ejmm.journals.ekb.eg/article_390574.html

Detail API

http://journals.ekb.eg?_action=service&article_code=390574

Order

17

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

MicroRNA 29 gene Expression and Progesterone Receptor Values in Iraqi Women with Breast Cancer

Details

Type

Article

Created At

28 Dec 2024