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388762

Evaluation of the Antagonistic Activity of Lactobacillus strains against Pathogenic Organisms isolated from Various Infected Wounds: An In Vitro Study

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: With the serious global threat of antimicrobial resistance, finding therapeutic alternatives has become a critical priority. Probiotics appear to be promising treatments due to their known natural inhibitory effects on various pathogens. Objectives: To evaluate the potential antagonistic effects of the probiotic Lactobacillus plantarum and Lactobacillus rhamnosus as well as their cell-free supernatant (CFS) and the synergistic interaction with commercial prebiotics like Fructooligosaccharides (FOS) on different pathogens isolated from infected wounds. Methodology: Identification of the pathogenic microorganisms was done. The tissue culture plate method evaluated the biofilm formation ability among the isolated pathogens.  The antimicrobial activity of L. plantarum and L. rhamnosus and their CFS were investigated by radial streak technique and agar well diffusion. The anti-biofilm effect of CFS of lactobacilli was evaluated using tissue culture plate assay. Results: Seventy-two isolates were identified, 73.6% were Gram-negative, and the most prevalent was Klebsiella pneumoniae. Biofilm formation was identified in 69.5 % of the isolates, among them E. coli, Acinetobacter baumannii, and Proteus mirabilis were the most frequent biofilm-forming organisms. L. plantarum, L. rhamnosus and their CFS exhibited antimicrobial and antibiofilm activity against all tested pathogens with variable degrees. The addition of FOS enhanced both lactobacilli's antimicrobial and antibiofilm properties. Conclusion: The probiotics used showed significant antagonistic activity against various pathogens paving the way to be used as a new treatment option.

DOI

10.21608/ejmm.2024.326234.1352

Keywords

lactobacillus, Fructooligosaccharides, probiotic, prebiotic

Authors

First Name

Eman

Last Name

Hegazy

MiddleName

E.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

Email

eman.hegazi@med.tanta.edu.eg

City

Tanta

Orcid

0000-0001-9263-1894

First Name

Mona

Last Name

Gadallah

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

Email

mona.gadallah@med.tanta.edu.eg

City

Tanta

Orcid

-

First Name

Tarek

Last Name

Shoukr

MiddleName

G.

Affiliation

Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Tanta University

Email

tarek.shokr@med.tanta.edu.eg

City

Tanta

Orcid

-

First Name

Marwa

Last Name

Bahey

MiddleName

G.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

Email

marwabahey@gmail.com

City

Tanta

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-10-13

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_388762.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=388762

Order

388,762

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluation of the Antagonistic Activity of Lactobacillus strains against Pathogenic Organisms isolated from Various Infected Wounds: An In Vitro Study

Details

Type

Article

Created At

28 Dec 2024