Beta
ManageSearchChatDiscover
380824

Prevalence of Carbapenem Resistance and Antibacterial Potential of Nanoparticles in Pseudomonas aeruginosa Isolated from Burn Units in Egypt

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Pseudomonas aeruginosa is an opportunistic microorganism and quite frequently associated with skin infections. Biosynthesized silver nanoparticles can be a promising key to eliminate these microbes. Objectives: The current work aims to identify P. aeruginosa carrying metallo-β-lactamase from burn infections and assess the impact of silver nanoparticles and antibiotics on them. Methods: A total of 120 samples collected from patients suffering from burn wound infections were subjected to conventional microbiological techniques to identify P. aeruginosa isolates. Testing for antimicrobial susceptibility was conducted utilizing the Kirby Bauer disc diffusion method. Carbapenemase-producing strains were detected both phenotypically by detecting metallo-beta-lactamases through performing modified carbapenem inactivation method (mCIM) and genotypically using PCR. Using the agar well diffusion method, AgNPs' antibacterial activity was assessed. The synergistic effect of a combination of antimicrobials (colistin, gentamicin, and ciprofloxacin) and AgNPs was estimated by the checkerboard method. Results:Out of 120 isolates, 46 (38%) P. aeruginosa isolates were identified and confirmed by PCR assay. Total 25 isolates (54%) were multidrug resistant isolates. Modified carbapenem inactivation method showed that 39 isolates (84.78%) were producing carbapenemases whereas 35 isolates (76%) were confirmed for carbapenemases by performing PCR. The prevalence of carbapenemase encoding genes was as follows blaSPM (14%), blaVIM (25.7%), blaNDM (40%), blaKPC (2.85%) and blaIMP (17%). Conclusions: P. aeruginosa isolates showed high-level carbapenem resistance. The majority of the isolates were multi drug resistant (MDR), indicating a concerning spread of resistant isolates. AgNPs exhibited considerable antibacterial effects, so they should be  considered as an effective means of combating MDR.

DOI

10.21608/ejmm.2024.317144.1325

Keywords

P. aeruginosa, Carbapenemase production, AgNPs

Authors

First Name

Rasha

Last Name

Mostafa

MiddleName

G.

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt

Email

rashagalal2009@yahoo.com

City

-

Orcid

-

First Name

Amany

Last Name

El fakhrany

MiddleName

-

Affiliation

Department of Clinical pharmacology, Faculty of Medicine, Menoufia University, Egypt

Email

amany.amany@med.menofia.edu.eg

City

-

Orcid

-

First Name

Fatma

Last Name

Ahmed

MiddleName

-

Affiliation

Department of Clinical pharmacology, Faculty of Medicine, Menoufia University, Egypt

Email

fatma.shehata@med.menofia.edu.eg

City

-

Orcid

-

First Name

Heba

Last Name

Abd el kareem

MiddleName

-

Affiliation

Department of Biochemistry, Molecular Biology and Physiology. Mutah Faculty of Medicine. Mutah University' Jordan

Email

drhebakareem@mutah.edu.jo

City

-

Orcid

-

First Name

Asmaa

Last Name

Soliman

MiddleName

-

Affiliation

biochemistry, Shebin el kom teaching hospital, Egypt

Email

asmaamohamedsoliman93@gmail.com

City

-

Orcid

-

First Name

Halal

Last Name

Khalil

MiddleName

-

Affiliation

Department of Clinical Microbiology and Immunology, National liver institute, Menoufia University, Egypt

Email

halaabazeed@gmail.com

City

-

Orcid

-

First Name

As

Last Name

Sharfeldin

MiddleName

-

Affiliation

Department of Public Health and Community medicine, Faculty of Medicine, Menoufia University, Egypt

Email

asmaasharfeldin@med.menofia.edu.eg

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-08-31

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_380824.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=380824

Order

380,824

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Prevalence of Carbapenem Resistance and Antibacterial Potential of Nanoparticles in Pseudomonas aeruginosa Isolated from Burn Units in Egypt

Details

Type

Article

Created At

28 Dec 2024