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379778

Detection of OqxA and OqxB Efflux Pump Genes in Multidrug Resistant Klebsiella Pneumoniae Isolated from Intensive Care Unit Patients

Article

Last updated: 20 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Objective: Detection of OqxA and OqxB efflux pump genes in multidrug resistant Klebsiella pneumoniae. Methodology: This study was done on 80 Klebsiella pneumoniae isolates from patients in Intensive Care Unit of Benha University Hospitals. The isolated Klebsiella pneumoniae was identified by conventional laboratory methods with subspecies identification by VITEK 2 system. Also, Antibiotic sensitivity tests were done by VITEK 2 method. OqxA and OqxB genes were detected by PCR. Results: Ninety percentages of isolates were carrying OqxA gene and 60% of isolates were carrying OqxB gene while OqxA and OqxB genes together were in 60% of isolated K. Pneumoniae. The OqxA gene was detected in 94.1% and OqxB was detected in 67.6% of isolated multidrug resistant Klebsiella pneumoniae. Conclusion: There is high correlation between OqxAB efflux pump genes and resistance to different antibiotic classes including quinolones and fluoroquinolone antimicrobial agents. This study shows that there is a significant association between the existence of OqxAB genes and MDR Klebsiella pneumoniae.

DOI

10.21608/ejmm.2024.316582.1319

Keywords

Klebsiella pneumoniae, multidrug resistance, efflux pump, OqxAB

Authors

First Name

Mohamed

Last Name

Awadallah

MiddleName

G.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Benha University

Email

dr_mohamed4156@gmail.com

City

-

Orcid

-

First Name

Somaya

Last Name

Desouky

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Benha University

Email

dr.somaya4567@gmail.com

City

-

Orcid

-

First Name

Mona

Last Name

Saleh

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Benha University

Email

monasaleh898989@gmail.com

City

-

Orcid

-

First Name

Marwa

Last Name

EL-Melouk

MiddleName

S.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Benha University

Email

dr.marwaseif@gmail.com

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-08-29

Publish Date

2025-01-01

Page Start

39

Page End

44

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 1_Pages 39-44.pdf

PDF . 521.4kB

Link

https://ejmm.journals.ekb.eg/article_379778.html

Detail API

http://journals.ekb.eg?_action=service&article_code=379778

Order

5

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of OqxA and OqxB Efflux Pump Genes in Multidrug Resistant Klebsiella Pneumoniae Isolated from Intensive Care Unit Patients

Details

Type

Article

Created At

28 Dec 2024