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378540

A Study of Accessory Gene Regulator, Biofilm Formation, and Antibiotics Resistance of Staphylococcus aureus Isolated from Pediatric Patients with Healthcare-Associated Sepsis

Article

Last updated: 28 Dec 2024

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Tags

Clinical microbiology

Abstract

Background: Among pediatric patients, Staphylococcus aureus (S. aureus) is a significant bacterium that causes healthcare-associated sepsis. Antibiotic resistance and biofilm development, a virulence factor in S. aureus, pose significant challenges to infection control. The accessory gene regulator (agr) is a regulatory gene believed to control multiple factors of virulence in Staphylococcus aureus. Objective: To investigate the ability of isolated S. aureus from pediatric patients with sepsis to produce biofilms and explore different types of agr systems and their interrelationships. Methodology: In this work, 115staph. aureus strains isolated from positive blood cultures from pediatric patients diagnosed with sepsis were tested for biofilm production, resistance to antibiotics and different types of agr  genes by PCR. Results: Out of 115 of S. aureus strains, 91.3% of the isolates were capable of forming biofilm. We observed a significant biofilm formation capacity in 52 strains (49.5%), a moderate capacity in 31 strains (29.5%), and a weak capacity in (20.9%). PCR revealed that the agrI and agrIII genes were the most prevalent, with prevalence rates of 41.7% and 25.2 respectively, while the agrII gene was the least common at 9.6%, respectively. Also we reported a strong link between the placement of peripheral catheters and genotype Agr II. Also we reported a strong link between genotype agr II and the growth of robust biofilm. Conclusion: S. aureus forms a strong biofilm in pediatric patients with healthcare-associated sepsis which indicates virulence and difficulty in its eradication by antibiotics therapy. Agr II showed a positive association with the formation of strong biofilms.

DOI

10.21608/ejmm.2024.312585.1305

Keywords

S. aureus, agr genes, Biofilm, antibiotics resistance, PCR

Authors

First Name

Mohamed

Last Name

Rizk

MiddleName

A.

Affiliation

Clinical Pathology, Faculty of Medicine, Mansoura University-Mansoura, Egypt

Email

mohamedanniesm@jotmail.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Bakr

MiddleName

-

Affiliation

Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

bakr_as@hotmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Elsayed

MiddleName

G.

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

gomaah@gmail.com

City

-

Orcid

-

First Name

Radwa

Last Name

Rabea

MiddleName

A.

Affiliation

Clinical Pathology, Faculty of Medicine Beni suef University, Beni suef, Egypt

Email

rabeae2@hotmail.com

City

-

Orcid

-

First Name

Maysaa

Last Name

Zaki

MiddleName

E.

Affiliation

Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

may_s@mans.edu.eg

City

-

Orcid

-

First Name

Mai

Last Name

Ahmed

MiddleName

E.

Affiliation

Clinical Pathology, Faculty of Medicine Beni suef University, Beni suef, Egypt

Email

dr.menoo@hotmail.com

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

50252

Issue Date

2025-01-01

Receive Date

2024-08-29

Publish Date

2025-01-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_378540.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=378540

Order

378,540

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

A Study of Accessory Gene Regulator, Biofilm Formation, and Antibiotics Resistance of Staphylococcus aureus Isolated from Pediatric Patients with Healthcare-Associated Sepsis

Details

Type

Article

Created At

28 Dec 2024