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384990

Unveiling the Microbial Landscape: Chronic Airway Colonization in Egyptian Children with Cystic Fibrosis

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Cystic fibrosis (CF) is a genetically recessive disease marked by frequent, chronic lung infections that impair lung function and cause early death. Objectives: our aim was to identify the bacteria colonizing the airways among a cohort of Egyptian children with CF and to assess their antimicrobial susceptibility and biofilm formation capacity. Methodology: In this cross-sectional study, we assessed the prevalence of bacterial species colonizing airways of 34 Egyptian pediatric patients with CF. The isolated pathogens underwent antibiotic susceptibility testing using the Kirby–Bauer disc diffusion method and biofilm formation analysis via a microtiter plate assay. Results: In a total of 43 isolated pathogens, the most frequently isolated microorganisms were Staph aureus (S. aureus) (51.2%) followed by Pseudomonas aeruginosa (P. aeruginosa) (34.9%). Other less prevalent bacterial isolates included E. coli, Klebsiella spp., and Acinetobacter spp. (4.7% each). Regarding antibiotic susceptibility profiles, S. aureus demonstrated high resistance to penicillin and cefoxitin (95.5% and 81.8% respectively) followed with erythromycin and tetracycline, with resistance rates of 68.2% and 45.5% respectively. Alarmingly, a high prevalence of Methicillin-Resistant S. aureus (MRSA) was observed among our tested isolates (81.8%). Among P. aeruginosa isolates, cefepime and imipenem exhibited the highest resistance rates (26.7% each), followed by tobramycin (20%). All isolated pathogens produced biofilm with varying degrees. Among them 50% of S. aureus and 66.7% of P. aeruginosa were strong biofilm producers. Conclusion: There was a significantly higher prevalence of S. aureus chronic colonization among our CF patients. Meanwhile chronic colonization with P. aeruginosa was less prevalent. Unfortunately, there was a high prevalence of MRSA and biofilm formation among the isolated bacteria. Implementing strict preventive measures and infection control can help prevent MRSA-related exacerbations in these patients.

DOI

10.21608/ejmm.2024.325869.1351

Keywords

Antibiotics susceptibility test, biofilm formation, Cystic fibrosis, Pseudomonas aeruginosa, Staphylococcus aureus

Authors

First Name

Mariam

Last Name

Karas

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

drmariamkaras@gmail.com

City

-

Orcid

-

First Name

Erini

Last Name

Fawzy

MiddleName

F.

Affiliation

Pediatrics Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

erinifaridfawzy@gmail.com

City

-

Orcid

-

First Name

Dalia

Last Name

ElFeky

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

dselfeky@cu.edu.eg

City

-

Orcid

0000-0003-3633-3977

First Name

Zeinab

Last Name

Mostafa

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

zeinabmostafa61@gmail.com

City

-

Orcid

-

First Name

Hagar

Last Name

Mowafy

MiddleName

L.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

hagarmowafy@kasralainy.edu.eg

City

Giza

Orcid

0000-0003-3837-5978

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-10-03

Publish Date

2024-10-01

Page Start

239

Page End

247

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 239-247.pdf

PDF . 457.1kB

Link

https://ejmm.journals.ekb.eg/article_384990.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=384990

Order

384,990

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Unveiling the Microbial Landscape: Chronic Airway Colonization in Egyptian Children with Cystic Fibrosis

Details

Type

Article

Created At

28 Dec 2024