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380315

Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Juvenile onset systemic lupus erythematous (JSLE) and the accompanying lupus nephritis (LN) (affects up to 80% of JSLE) harbor more aggressive disease courses and major complications with higher morbidity and mortality risks compared to adult onset SLE. Two microRNAs (miRNAs), miR-130b-3p and miR-148a-3p, are strong candidates in the pathogenesis of both JSLE and LN. Objective: Studying serum expression of miR-130b-3p and miR-148a-3p patients with JSLE and LN to evaluate their contribution to their pathogenesis. Methodology: The study included 100 LN, 50 JSLE and 50 healthy controls (HC), reverse transcriptase real time PCR (RT-QPCR) was used for evaluation of serum levels of miR-130b-3p and miR-148a-3p. Results: miR-130b-3p showed decreased expression in JSLE and LN patients compared to HC and correlated inversely with serum creatinine within patients' groups. miR-148a-3p significantly decreased expression in JSLE patients compared to controls and LN patients. MiR-148a-3p was elevated in LN cases compared to JSLE but not HC showing inverse correlation with duration of LN and serum creatinine. Positive correlation between miR-130b-3p and miR-148a-3p was detected in diseased groups (r = 0.75, p = 0.001). Conclusion: Our study was the first to analyze both miR-130b-3p & miR-148a-3p expression in JSLE and LN and we concluded that both might affect the pathogenesis of JSLE and that miR-148a-3p may significantly contribute to the pathogenesis of LN among JSLE patients.

DOI

10.21608/ejmm.2024.318532.1330

Keywords

Keywords: SLE, Juvenile onset systemic lupus erythematous, Lupus nephritis, miR-130b-3p, miR-148a-3p

Authors

First Name

Ingy

Last Name

Ashmawy

MiddleName

-

Affiliation

Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

ingyashmawy@ymail.com

City

Giza

Orcid

0000-0002-6598-3568

First Name

Abeer

Last Name

Ramadan

MiddleName

-

Affiliation

Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Institute, National Research Centre, Cairo, Egypt

Email

dr_aramadan1978@yahoo.com

City

Cairo, Egypt

Orcid

-

First Name

Neveen

Last Name

Helmy

MiddleName

A.

Affiliation

Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

naivenhelmy@hotmail.com

City

-

Orcid

0000-0002-6535-4331

First Name

Huda

Last Name

Marzouk

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

hoda.hassan@kasralainy.edu.eg

City

-

Orcid

-

First Name

Yomna

Last Name

Farag

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

yomna.farag@kasralainy.edu.eg

City

-

Orcid

-

First Name

Shereen

Last Name

Abd El Aziz

MiddleName

H.

Affiliation

Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

sherryhamdy4@yahoo.com

City

-

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-09-05

Publish Date

2024-10-01

Page Start

221

Page End

226

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 221-226.pdf

PDF . 341.0kB

Link

https://ejmm.journals.ekb.eg/article_380315.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=380315

Order

27

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis

Details

Type

Article

Created At

28 Dec 2024