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380314

Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Host response

Abstract

Background: Sepsis is a life- threatening organ dysfunction caused by impaired host response to infection. Objectives: To assess the immune status in children with sepsis and septic shock using mRNA gene expression of 7 biomarkers; Interleukin (IL)-1ß, Tumor necrosis factor (TNF), IL-10, Programmed death protein (PD-1) and Programmed death ligand 1(PD-L1), BID (inhibitory check point), and BCL2 (anti-apoptotic). Secondly, to study the possible influence of G-CSF on the expression of these biomarkers and outcome in septic children. Methodology: This is a case controlled prospective randomized biomarker study including 65 subjects divided into 3 groups. Group 1 comprised 24 septic patients receiving G-CSF in addition to conventional treatment for sepsis. Group 2 included 17 septic patients on conventional treatment. Group 3 control group comprised 24 infants and children matched for age and sex. Two blood samples were withdrawn from each patient at day 1 and day 8. RT-PCR analysis for mRNA gene expression of the studied biomarkers were performed. Results: Group 1 showed significant increase of PD-L1 on day 8 compared to day 1 (p= 0.019) and significant increase of BID1 on day 8 (p= 0.014) and on day 1 (p= 0.08) compared to healthy children. In group 2, IL-10 showed significant decrease on day 8 compared to day 1(p= 0.02). BID1 showed highly significant increase between both days 1 and 8 versus healthy children (p = 0.001, 0.004 respectively). BCL-2 showed significant decrease in serum levels between both days 1 and 8 versus healthy children (p = 0.04 ,0.012 respectively). Conclusion: Upregulation of the apoptotic BID1 and PD-L1 expression with downregulation of IL-10 and BcL2 expression in our septic patients suggests a feature of sepsis-related immunosuppression. G-CSF had no role on the studied biomarkers release and outcome.

DOI

10.21608/ejmm.2024.319256.1334

Keywords

apoptotic BID1, PD-L1, Bcl2, Cytokines, Sepsis

Authors

First Name

Sally

Last Name

EL-Sahrigy

MiddleName

A. F.

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

sallyelsahrigy@yahoo.co.uk

City

-

Orcid

-

First Name

Tarek

Last Name

Abdel Gawad

MiddleName

A.

Affiliation

Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Email

gawad57@hotmail.com

City

-

Orcid

-

First Name

Azza

Last Name

Abdel Rahman

MiddleName

M. O,

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

azzaomar_74@yahoo.com

City

-

Orcid

-

First Name

Sondos

Last Name

Magdy

MiddleName

-

Affiliation

Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Email

sondosmmagdy@gmail.com

City

-

Orcid

-

First Name

Eman

Last Name

Mostafa

MiddleName

A.

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

dremyali@yahoo.com

City

-

Orcid

-

First Name

Rasha

Last Name

Hasanin

MiddleName

M.

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

rashamahmoud97@yahoo.com

City

-

Orcid

-

First Name

Ashraf

Last Name

Galal

MiddleName

-

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

ashrafgalal150@yahoo.com

City

-

Orcid

-

First Name

Hanan

Last Name

Hamed

MiddleName

M.

Affiliation

Pediatrics Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

hmhamed63@yahoo.com

City

-

Orcid

-

First Name

Eman

Last Name

Hassan

MiddleName

M.

Affiliation

Clinical and Chemical Pathology Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

dr.eman.attia@hotmail.com

City

-

Orcid

-

First Name

Amany

Last Name

Abdelrahman

MiddleName

H.

Affiliation

Clinical and Chemical Pathology Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

amanyhosny123@yahoo.com

City

-

Orcid

-

First Name

Mirhane

Last Name

Hassan

MiddleName

-

Affiliation

Clinical and Chemical Pathology Department, Medical Research. and Clinical Studies Institute, National Research Centre, Cairo, Egypt

Email

mirhanehassan@gmail.com

City

-

Orcid

0000-0001-6193-9415

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-09-08

Publish Date

2024-10-01

Page Start

211

Page End

219

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 211-219.pdf

PDF . 529.8kB

Link

https://ejmm.journals.ekb.eg/article_380314.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=380314

Order

26

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Immune Status Assessment using mRNA gene Expression of New Biomarkers in Children with Sepsis and Septic Shock: Role of G-CSF in Improving the Outcome

Details

Type

Article

Created At

28 Dec 2024