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378995

Assessment of Serum MicroRNA-221-3p and Tumor Necrosis Factor-α Levels in Patients with Chronic Plaque Psoriasis: A case-control Study

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Chronic plaque psoriasis (CPP) is the most prevalent subtype of psoriasis, which is a chronic inflammatory disorder. MicroRNAs (miRNAs) may contribute to psoriasis pathogenesis through regulation of cytokine production. The pro-inflammatory cytokine, tumor necrosis factor-α (TNF-α), exerts a central role in psoriasis pathogenesis. Objective: to compare serum levels of miR-221-3p and TNF-α among CPP patients and healthy individuals. Methodology: 45 CPP patients and 45 healthy participants were included. The Psoriasis Area and Severity Index (PASI) score was utilized to assess the disease severity. Quantitative real-time PCR and ELISA techniques were used for assessment of serum concentrations of miRNA-221-3p and TNF-α, respectively. Results: Psoriatic patients showed significantly higher serum concentrations of miR-221-3p and TNF-α than healthy controls (P < 0.001). Moreover, a significant direct correlation was detected between serum miR-221-3p and TNF-α concentrations, on one hand, and both PASI score and disease duration (P < 0.001). Furthermore, a significant direct correlation was detected between concentrations of miR-221-3p relative expression and TNF-α in the sera of psoriatic cases (r = 0.876, P < 0.001). Conclusion: The miR-221-3p/TNF-α interaction may contribute to psoriasis pathogenesis. Moreover, miR-221-3p could be utilized as a biomarker to diagnose psoriasis and to assess its severity.

DOI

10.21608/ejmm.2024.313912.1313

Keywords

MiRNA -221-3p, pathogenesis, Psoriasis, serum, Tumor necrosis factor

Authors

First Name

Rehab

Last Name

Tash

MiddleName

M.E

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

relsaid193@yahoo.com

City

Zagazig

Orcid

-

First Name

Zahira

Last Name

Elsayed

MiddleName

M.F.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

miro19377@gmail.com

City

-

Orcid

-

First Name

Nashwa

Last Name

Selim

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

nashwaselim529@gmail.com

City

-

Orcid

-

First Name

Manal

Last Name

Fawzy

MiddleName

M.

Affiliation

Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

manal.saraha@gmail.com

City

-

Orcid

-

First Name

Yasmin

Last Name

Fahmy

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

yasminfahmy@yahoo.com

City

Zagazig

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-23

Publish Date

2024-10-01

Page Start

195

Page End

202

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 195-202.pdf

PDF . 452.9kB

Link

https://ejmm.journals.ekb.eg/article_378995.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=378995

Order

24

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Assessment of Serum MicroRNA-221-3p and Tumor Necrosis Factor-α Levels in Patients with Chronic Plaque Psoriasis: A case-control Study

Details

Type

Article

Created At

28 Dec 2024