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377930

Flowcytometric Expression of CD11b and Detection of Netosis Markers (Calprotectin, Protease 57 and PADI4) in Women with RPL at Basrah Province

Article

Last updated: 05 Jan 2025

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Subjects

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Tags

typing and identification

Abstract

Background: pregnancy is defined as a special immunological condition when the mother's immune system protects the fetus against infection. Objective:  the current study is designed to evaluate the role and expression of NETosis markers (Calprotectin, Protease 57 and PADI4) by ELISA and CD11b by Flowcytometry in women with Recurrent Pregnancy Loss (RPL) at Basrah Province. Methodology: this prospective cohort study was conducted from January 2022 to July 2023, included 75 participants (50 RPL patients and 25 controls). Blood samples were analyzed using ELISA to measure the expression of Protease 57, Calprotectin, and Peptidyl Arginine Deiminase Type IV (PADI4), also the surface expression of CD11b on activated neutrophils was assessed via flow cytometry. Results: calprotectin demonstrated a significant increase, with mean values of 5.43 in RPL patients versus 4.66 in controls (p=0.021). Protease 57 exhibited significantly elevated with mean levels in the RPL group (3.28) compared with controls (1.15, p=0.0001). CD11b expression, assessed via flow cytometry was revealed significant upregulation in RPL patients. The mean fluorescence intensity of CD11b was 17.55, substantially higher than the 11.43 observed in controls (p=0.0001). Conclusion: the findings of this study provide significant insights into the intricate relationship between recurrent miscarriages and the immune system, specifically through the lens of neutrophil extracellular traps (NETs) and the activation marker CD11b.And further elucidates the underlying immune mechanisms. Notably, the investigation into NETosis markers, alongside the assessment of PADI4, calprotectin, and Protease 57 levels, illuminates the complex interplay of inflammatory responses and neutrophil activation in the context of miscarriage.

DOI

10.21608/ejmm.2024.314405.1315

Keywords

RPL, Calprotectin, Protease 57, PADI4, CD11b

Authors

First Name

Sally

Last Name

Abed

MiddleName

Sh.

Affiliation

Department of Food Science, Agriculture College, University of Basrah University

Email

mustafafadil837@gmail.com

City

Basra

Orcid

-

First Name

Wafaa

Last Name

Shani

MiddleName

S.

Affiliation

Department of Biology, College of Science, Basrah University

Email

wafaasshani@gmail.com

City

Basra

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-21

Publish Date

2024-10-01

Page Start

177

Page End

185

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 177-185.pdf

PDF . 470.5kB

Link

https://ejmm.journals.ekb.eg/article_377930.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=377930

Order

22

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Flowcytometric Expression of CD11b and Detection of Netosis Markers (Calprotectin, Protease 57 and PADI4) in Women with RPL at Basrah Province

Details

Type

Article

Created At

28 Dec 2024