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376534

Antibacterial activity of Solenostemma argel extracts against extended-spectrum β-lactamase producing Klebsiella pneumoniae

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Extended-spectrum β-lactamases (ESBLs) can hydrolyze various types of β-lactam antibiotics. Therefore, infections caused by ESBL-producing bacteria pose a significant challenge to clinical patient care. Objective: This study aimed to assess the prevalence of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) and evaluate the antibacterial potential of Solenostemma argel extracts against these isolates. Methodology: 200 clinical Klebsiella spp. isolates were identified by MALDI-TOF, and the Vitek2 system determined the antibiograms of K. pneumoniae. Phenotypic detection of ESBL was conducted using CHROMagar ESBL and the molecular characterization of blaTEM, blaSHV, and blaCTX-M genes was carried out by polymerase chain reaction (PCR). In addition, methanolic and ethanolic extracts of S. argel were prepared and their antibacterial activity was investigated. Results: K. pneumoniae isolates exhibited remarkable resistance to cefuroxime (83.9%), ciprofloxacin (58.3%), ceftazidime (55.3%), aztreonam (44%), and cefepime (36.3%), respectively. Moreover, results of the phenotypic detection of ESBL revealed that 28.5% of K. pneumoniae isolates produced ESBLs and the molecular characterization showed that 100, 87.5, and 45.8% of ESBL-Kp isolates were positive for blaSHV, blaCTX-M and blaTEM genes, respectively. Furthermore, the methanolic extract of S. argel exhibited promising antioxidant and antibacterial activity against ESBL-Kp with minimum inhibitory concentration (MIC) values ranged from 1 to 16 µg/mL. Conclusion: This investigation shed light on the elevated levels of resistance among clinical K. pneumoniae isolates accompanied by the acquisition of the blaSHV gene and verified the potent antibacterial activity of S. argel against ESBL-producing K. pneumoniae, suggesting the potential application of its methanolic extract in combating ESBL-Kp.

DOI

10.21608/ejmm.2024.313255.1309

Keywords

ESBLs, Klebsiella species, Antibiotic resistance, antimicrobial activity, Solenostemma argel

Authors

First Name

Sherin

Last Name

Sharaf

MiddleName

M.A.

Affiliation

Department of Microbiology, Egyptian Drug Authority (EDA), Giza, Egypt

Email

sherinashraf28@gmail.com

City

-

Orcid

0000-0003-0041-1822

First Name

Hanaa

Last Name

Ali

MiddleName

T.A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

hanaatoson@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Salah

MiddleName

G.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

farahat@sci.cu.edu.eg

City

-

Orcid

0000-0002-0872-0024

First Name

Zeinat

Last Name

Kamel

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

zeinatk@yahoo.com

City

-

Orcid

0000-0002-5013-2357

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-19

Publish Date

2024-10-01

Page Start

143

Page End

150

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 143-150.pdf

PDF . 443.7kB

Link

https://ejmm.journals.ekb.eg/article_376534.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=376534

Order

19

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Antibacterial activity of Solenostemma argel extracts against extended-spectrum β-lactamase producing Klebsiella pneumoniae

Details

Type

Article

Created At

28 Dec 2024