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376267

Phylogenetic Group of Escherichia coli Isolated from Inflammatory Bowel Disease in Al Najaf province

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Inflammatory Bowel Disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract that includes Crohn's disease (CD) and ulcerative colitis. Recent studies have highlighted the role of specific bacterial species in the pathogenesis of IBD, including adherent-invasive Escherichia coli (AIEC). Objectives: The study was designed to determine the prevalence of Crohn's disease compared with ulcerative colitis, and determination the predominant bacterial species causing inflammatory bowel disease in Al-Najaf city, Additionally the study focused on molecular detection of the phylogenetic classification of E. coli by PCR. Methodology: The study collected 102 stool specimens were collected from inflammatory bowel disease patients . Escherichia coli isolates were diagnosed identified by culture and biochemical reactions. Vitek-2 compact system  provides an automated final identification . the PCR approach was used for detecting the phylogenic groups by targeting three marker genes chuA and yjaA and DNA fragment TSPE4.C2. Result: Eighty eight isolates out of 102 (88%) were presumptively identified as Escherichia coli and 6 (5.88%) isolates identified as Pseudomonas spp and Klebsiella pneumoniae while 8 (7.84%) were negative growth. There were 90 cases diagnosed as Crohn's disease and only 12 cases diagnosed as ulcerative colitis. According to the results of PCR-based phylotyping, Phylogenetic group B2 (81.8%) was the most widely dispersed phylogenetic group of E. coli, followed by Phylogenetic group A (13.6%) and Phylogenetic group D (0.04%). Conclusion: Crohn's disease has a higher prevalence than ulcerative colitis. Phylogenetic group B2 Escherichia coli plays a significant role in inflammatory bowel disease pathogenesis by colonizing the gut and causing inflammation.

DOI

10.21608/ejmm.2024.307468.1293

Keywords

IBD, E. coli, Phylogroup, Crohn's Disease

Authors

First Name

Hiba

Last Name

Majeed

MiddleName

T.

Affiliation

Department of Vision screening Techniques, College of Health and Medical techniques, Al-Forat Al-Awsat University Kufa

Email

hibatakleef5555@yahoo.com

City

najaf

Orcid

-

First Name

Zahraa

Last Name

Motaweq

MiddleName

Y.

Affiliation

Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq

Email

zahraa.mutawak@uokufa.edu.iq

City

najaf

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-10

Publish Date

2024-10-01

Page Start

129

Page End

134

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 129-134.pdf

PDF . 497.7kB

Link

https://ejmm.journals.ekb.eg/article_376267.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=376267

Order

17

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phylogenetic Group of Escherichia coli Isolated from Inflammatory Bowel Disease in Al Najaf province

Details

Type

Article

Created At

28 Dec 2024