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376265

PCR Detection of Spreading TEM and CTX-M gene in Klebsiella oxytoca isolate from Urinary Tract Infection

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: urine is usually free of bacterial infection occurs by ascending or descending infection pathway. CTX-M enzymes are a group of class A extended-spectrum β-lactamases (ESBLs) that are rapidly spreading among Enterobacteriaceae, Most of the ESBLs are mutants of the TEM and the SHV enzymes and developed from point mutation of genes that code production of primordial TEM-1, TEM-2, or SHV-1 β lactamase enzymes. Objectives: This study aimed to detect K. oxytoca bacteria in urine samples obtained from individuals with urinary tract infections.and, we aimed to the existence of blaCTX-M and blaTEM genes in Klebsiella oxytoca in Najaf governorate from November 2022 to June 2023. Methodology: The study included 540 urine specimens. Based on culture and biochemical features, K. oxytoca isolates were identified. The Vitek-2 compact system provides a confirmatory test, PCR was used for detecting the blaCTX-M and blaTEM genes. Result:seventeen isolates were identified as K. oxytoca. Vitek further confirmed this, PCR technique targeting the blaCTX-M gene detected in 14 isolates, while 4 from the 17 isolates were positive for the blaTEM gene.Conclusion: The molecular study revealed that blaCTX-M and blaTEM gene were detected in (82.3%) and (23.5%) of K. oxytoca isolates carrying blaCTX-M and blaTEM genes respectively.

DOI

10.21608/ejmm.2024.306811.1287

Keywords

Klebsiella oxytoca, Urinary tract infection (UTI), CTX-M, and TEM

Authors

First Name

Duha

Last Name

Kashkool

MiddleName

A.H.

Affiliation

Department of Biology, Faculty of Science, University of Kufa, Iraq

Email

duhaalihasan22@yahoo.com

City

najaf

Orcid

-

First Name

Abbas

Last Name

Al-Muhanna

MiddleName

S.

Affiliation

Department of Biology, Faculty of Science, University of Kufa, Iraq

Email

abbasshakir33@yahoo.com

City

najaf

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-10

Publish Date

2024-10-01

Page Start

123

Page End

128

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 123-128.pdf

PDF . 424.2kB

Link

https://ejmm.journals.ekb.eg/article_376265.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=376265

Order

16

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

PCR Detection of Spreading TEM and CTX-M gene in Klebsiella oxytoca isolate from Urinary Tract Infection

Details

Type

Article

Created At

28 Dec 2024