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375100

Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) From Different Sources of Food in Basra Province / Iraq

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Staphylococcal food-borne illness (SFD) is a food-borne infection that is highly common around the world. MRSA is a major cause of infections and poses a significant global health threat due to the toxins it can produce in food. Objective: is to characterize S. aureus isolates from foodstuffs collected from Basrah. Methodology: A total of 135 food samples of locally made food products were collected from different areas of Basrah markets in February 2022. The samples included (milk, cake, cheese), and various types of meats (beef, chicken, lamb). Results and Discussion: showed the presence 27 strains of S. aureus in percentage of 20% of the diverse samples (135 samples). 10.7% of the meat samples, 6.2% of the milk samples, 1% of the cake samples, and 2.1% of the cheese samples all had S. aureus. Using the Polymerase Chain Reaction (PCR), a total of S. aureus isolates from different food products were collected and identified. The detection of the mecA gene identified MRSA isolates. PCR amplification of the 27 phenotypically S. aureus isolates revealed that 4 were found to carry mecA genes in a percentage of (14.81%), exhibited resistance to methicillin, and were identified as (MRSA). The following amplicon sizes were revealed by the gel electrophoresis results: (DNA fragments) 756 bp, 500 bp (16S rDNA gene of S. aureus), and 310 bp (mecA gene). Conclusion: our results suggest that artisanal food producers should have better control production hygiene measure because it represents a potential risk to public health.

DOI

10.21608/ejmm.2024.311434.1303

Keywords

SFD, MRSA, mecA genes, Staphylococcus aureus

Authors

First Name

Salah

Last Name

Aziz

MiddleName

N.

Affiliation

Department of Food Science, Agriculture College, University of Basrah, Basrah, Iraq

Email

mustafafadil837@gmail.com

City

Basra

Orcid

-

First Name

Khulood

Last Name

Huseein

MiddleName

A.

Affiliation

Department of Medical Science, Nursing College, University of Basrah, Basrah, Iraq

Email

-

City

-

Orcid

-

First Name

Ameer

Last Name

Mohammed

MiddleName

A.

Affiliation

Department of Marine Vertebrate, Marine Science Center, University of Basrah, Basrah, Iraq

Email

-

City

-

Orcid

-

First Name

Teif

Last Name

Najm

MiddleName

A.

Affiliation

Department of Biology, College of Education of Al Qurna, University of Basrah, Basrah, Ira

Email

-

City

-

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-20

Publish Date

2024-10-01

Page Start

91

Page End

97

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 91-97.pdf

PDF . 421.5kB

Link

https://ejmm.journals.ekb.eg/article_375100.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=375100

Order

12

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) From Different Sources of Food in Basra Province / Iraq

Details

Type

Article

Created At

28 Dec 2024