Beta
ManageSearchChatDiscover
374760

Identifying Colibactin-Producing Escherichia coli as Candidate Biomarker for Aggressive Colorectal Cancer

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Colorectal cancer represents the third most common cause of carcinogenesis and aggressive cancer cases have been linked to some pathogenic microorganisms, including colibactin-producing Escherichia coli. There are few biomarkers for colorectal cancer diagnosis and prognosis. However, there have been documented clinical challenges in differentiating between aggressive cancers and those that are not. Objectives: This study was designed to investigate the genetic diversity of E. coli strains of both colorectal carcinoma-associated and non-associated E. coli strains. Methodology: Pathogenic E. coli isolates from benign (24) and malignant (80) colorectal tissue biopsies were characterized based on bacteriological analysis. The Pks (polyketide synthase) Island, which specifically targets of E coli genes such as clbB and uidA was examined using polymerase chain reaction. Results: The colibactin gene (clbB) was found to be increased significantly in the malignant (62%) compared to benign (0%) colorectal tissues ( p<0.001). The presence of this gene was also associated significantly with high grade (p=0.042) and tumor size (p=< 0.001) of colorectal carcinoma. In contrast, there was no significant association between uidA gene and colorectal carcinoma clinical parameters. Conclusion: The aggressiveness and development of colorectal cancer may be related to the clbB gene. Further research is required to determine the role of this gene in colorectal carcinoma and it could be used as a biomarker to identify colorectal cancer.

DOI

10.21608/ejmm.2024.309274.1301

Keywords

Colibactin, clbB, uidA, E coli, Colorectal carcinoma

Authors

First Name

Oula

Last Name

Alalwany

MiddleName

S.

Affiliation

Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, 6400

Email

aula.s.msc@utq.edu.iq

City

Thi-Qar

Orcid

-

First Name

Dhafer

Last Name

Alghezi

MiddleName

A.

Affiliation

Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, 6400; Cancer Research Unit, College of Medicine, University of Thi-Qar, Thi-Qar, 6400

Email

dhafer.a.f.alghezi@bath.edu

City

-

Orcid

0000-0002-5450-6360

First Name

Rasha

Last Name

Aljawher

MiddleName

Q.

Affiliation

Department of Histopathology and Forensic Medicine, College of Medicine, University of Thi-Qar, Thi- Qar, 64001

Email

dr.rasha.jawher@jmail.com

City

Thi-Qar

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-08-04

Publish Date

2024-10-01

Page Start

59

Page End

66

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 59-66.pdf

PDF . 463.9kB

Link

https://ejmm.journals.ekb.eg/article_374760.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=374760

Order

8

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Identifying Colibactin-Producing Escherichia coli as Candidate Biomarker for Aggressive Colorectal Cancer

Details

Type

Article

Created At

28 Dec 2024