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Impacts of Polymerase Chain Reaction confirmed Herpes Simplex Virus type-1 Keratitis on patients attending Ophthalmology Center Mansoura University

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

virology

Abstract

Herpes simplex keratitis (HSK) is a significant corneal disease leading to visual impairment, with an estimated 1.8 million cases worldwide annually and over 230,000 new instances of monocular vision impairment reported in 2016, caused by the herpes simplex virus (HSV), particularly type 1 (HSV-1). HSK has a prolonged course and high recurrence rate, negatively impacting patients' lives and potentially causing blindness if untreated. Our study aimed to determine the proportion of confirmed HSV type 1 from total clinically suspected viral corneal infection at Ophthalmology Center, Mansoura University, and also to recognize associated risk factors that may increase the severity of the infection. We identified 23 positive cases out of 100 patients with suspected viral corneal infection by traditional PCR analysis targeting the DNA polymerase gene of HSV type-1. Our study did not reveal statistically significant associations between the evaluated risk factors and the occurrence or severity of HSK, but we observed a tendency for higher infection rates among specific demographics such as: patients in their fourth and fifth decades of life, males, and individuals residing in rural areas. In addition, univariate regression analysis showed that keratoplasty was the only predictor that had a statistically significant association with any poor outcome and recurrent infection. This study also revealed that corneal ulceration and scarring were the most common complications of HSK, necessitating urgent management to protect against potential vision loss.

DOI

10.21608/ejmm.2024.307934.1294

Keywords

Herpes simplex keratitis, HSV, Herpes Simplex Virus

Authors

First Name

Lamis

Last Name

Taha

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Egypt

Email

lamis_mohamed@mans.edu.eg

City

Mansoura

Orcid

-

First Name

Amr

Last Name

El-Sabbagh

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Egypt

Email

amrelsapagh@yahoo.com

City

Mansoura

Orcid

-

First Name

Ashraf

Last Name

Moawad

MiddleName

E.

Affiliation

Ophthalmology Center, Faculty of Medicine, Mansoura University, Egypt

Email

ashrafmoawad@yahoo.com

City

Mansoura

Orcid

-

First Name

Mona

Last Name

Abd Elraouf

MiddleName

A.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Egypt

Email

monaabdelraof@mans.edu.eg

City

Mansoura

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-07-28

Publish Date

2024-10-01

Page Start

39

Page End

46

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_372300.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=372300

Order

5

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Impacts of Polymerase Chain Reaction confirmed Herpes Simplex Virus type-1 Keratitis on patients attending Ophthalmology Center Mansoura University

Details

Type

Article

Created At

28 Dec 2024