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371103

Cytotoxicity and antibacterial activity of actinomycetes-mediated biogenic silver nanoparticles against methicillin-resistant Staphylococcus aureus (MRSA)

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: MRSA is one of the most life-threatening pathogens, thus its prevention has become a crucial public health concern. Objectives: This study aims to determine the prevalence of MRSA among coagulase-positive staphylococci and evaluate the cytotoxicity and anti-MRSA efficacy of biogenic silver nanoparticles (AgNPs). Methodology: Phenotypic detection of MRSA among 220 coagulase-positive Staphylococcus spp. isolates was investigated by CHROMagar™ MRSA, and their identification was confirmed by MALDI-TOF and detection of nuc and mecA genes. Furthermore, the actinomycetes-mediated biosynthesized AgNPs were characterized, and their cytotoxicity and anti-MRSA potential were assessed. Results: Of 220 isolates, 92 (41.8%) were presumptively identified as MRSA by CHROMagar MRSA, however, MALDI-TOF discriminated them into S. aureus, S. pseudintermedius, S. cornubiensis, and S. delphini. Results of molecular characterization revealed the presence of nuc and mecA genes in all confirmed MRSA isolates. In addition, a promising AgNPs-producing Streptomyces virginiae SNPPA6 was isolated, identified and utilized for extracellular biosynthesis of spherical AgNPs with an average size of 11.18 nm. The characterization of the AgNPs by XRD and FTIR revealed their crystalline nature and the presence of proteinaceous capping agents. The biogenic AgNPs exhibited promising anti-MRSA activity with MIC values ranging from 4 to 64 µg/mL. Cytotoxicity assessments using MTT assay indicated the cytotoxic impact of AgNPs on MDA-MB-231, A549 and HSF cell lines with IC50 values of 24.5, 29.2 and 36.3 µg/mL, respectively. Conclusion: Our findings highlighted the potential application of the biogenic AgNPs produced by S. virginiae SNPPA6 as a promising candidate for fighting MRSA at non-cytotoxic concentrations.

DOI

10.21608/ejmm.2024.307218.1290

Keywords

MRSA, Biosynthesis, silver nanoparticles, Cytotoxicity, antimicrobial activity

Authors

First Name

Fatma

Last Name

Sayed

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

tamtam_science@yahoo.com

City

-

Orcid

-

First Name

Mahmoud

Last Name

El-Rouby

MiddleName

N.

Affiliation

Egyptian National Cancer Institute, Cairo University, Cairo, Egypt

Email

lm1571760@gmail.com

City

-

Orcid

-

First Name

Zeinat

Last Name

Kamel

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

zeinatk@yahoo.com

City

-

Orcid

0000-0002-5013-2357

First Name

Mohamed

Last Name

Farahat

MiddleName

G.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt; Biotechnology Department, Faculty of Nanotechnology for Postgraduate Studies, Sheikh Zayed Branch Campus, Cairo University, Sheikh Zayed City

Email

farahat@cu.edu.eg

City

Giza

Orcid

0000-0001-8590-3652

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-07-27

Publish Date

2024-10-01

Page Start

21

Page End

28

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 21-28.pdf

PDF . 598.5kB

Link

https://ejmm.journals.ekb.eg/article_371103.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=371103

Order

3

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Cytotoxicity and antibacterial activity of actinomycetes-mediated biogenic silver nanoparticles against methicillin-resistant Staphylococcus aureus (MRSA)

Details

Type

Article

Created At

28 Dec 2024