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CD177, S100A8 and S100A12 as Markers of Severity in COVID- 19 Patients

Article

Last updated: 05 Jan 2025

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-

Tags

Clinical microbiology

Abstract

Background: Several important biomarkers associated with specific phases of the evolution of COVID-19 have been identified. CD177 is a specific neutrophil marker of activation, adhesion to the endothelium, and transmigration. The association of neutrophil activation signature with COVID-19 severity has also been described with CD177 gene being one of the most differentially expressed gene in advanced disease. S100 protein family are abundantly expressed by neutrophils. elevated expression levels of calprotectin (S100A8 and S100A12) was suggested to be associated with severe COVID-19 patients. Objectives: This study investigate the relation between gene expression of CD177, serum level of CD177, S100A8 and S100A12 and susceptibility to develop severe COVID-19. Method: This study included 60 COVID-19 patients who were hospitalized in the isolation hospital (30 severe and 30 non-severe) and 30 healthy volunteers as a control group.  For all study groups CD177 expression level was measured by RT-PCR and CD177, S100A8 and S100A12 serum level was measured by ELISA technique. Results: The mean serum levels of CD177 gene expression were much higher in severe than non-severe group (p = 0.001). Serum CD177 level was much higher in severe than non-severe group (p = 0.0004) and Serum S100A8 level was much higher in severe than non-severe group (p = 0.01). Our results show that  combined serum S100A8 and serum CD177 levels could predict the severity of COVID-19 infections with a sensitivity 84%, Specificity 87.5%, cut off value>162.8 ng/ml (p=0.001), and >84.7ng/ml(p<0.0001) respectively. Conclusion: Our results suggest that gene expression of CD177, serum level of CD177 and S100A8 were positively correlated with severe COVID 19 patients while that of serum level of S100A12 was not. Also combined serum S100A8 and serum CD177 levels could predict the severity of COVID-19 infections.

DOI

10.21608/ejmm.2024.295991.1259

Keywords

SARS-CoV-2, Covid-19 severity, CD177, S100A8, S100A12

Authors

First Name

Hala

Last Name

Ahmed

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Sohag University

Email

halaabdelaal8@gmail.com

City

-

Orcid

-

First Name

Abeer

Last Name

Sheneef

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Sohag University

Email

dr_ab_sh@yahoo.com

City

sohag

Orcid

-

First Name

Asmaa

Last Name

Goda

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Sohag University

Email

asmaagoda80@gmail.com

City

sohag

Orcid

0000-0002-2161-7267

First Name

Mai

Last Name

Hamed

MiddleName

H.

Affiliation

Specialist of Microbiology and Immunology, Faculty of Medicine, Elhelal Hospital

Email

maihamdy3535@gmail.com

City

sohag

Orcid

-

First Name

Amal

Last Name

Noreldin

MiddleName

K.

Affiliation

Department of Internal Medicine, Faculty of Medicine, Sohag University

Email

amalkhalifa0108@gmail.com

City

sohag

Orcid

-

First Name

Amira

Last Name

Ahmed

MiddleName

E.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Sohag University

Email

amiraesmail92@yahoo.com

City

sohag

Orcid

-

Volume

33

Article Issue

4

Related Issue

49337

Issue Date

2024-10-01

Receive Date

2024-06-09

Publish Date

2024-10-01

Page Start

1

Page End

9

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 4_Pages 1-9.pdf

PDF . 412.2kB

Link

https://ejmm.journals.ekb.eg/article_368782.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=368782

Order

1

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

CD177, S100A8 and S100A12 as Markers of Severity in COVID- 19 Patients

Details

Type

Article

Created At

28 Dec 2024