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Phenotypic And Genotypic Characterization Of Carbapenem – Resistant Gram – Negative Bacteria Among Pediatrics With Positive Blood Cultures

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Carbapenems are β-lactam antibiotics, which are regarded as one of the final options to treat severe infections resulting from multidrug-resistant organisms. Carbapenem resistance is mostly caused by carbapenemases, which are enzymes that break down this kind of β-lactam antibiotic. It can also occur through non-enzymatic processes such as efflux or changes in porin structure when AmpC or extended-spectrum β-lactamases (ESBL) are present. Carbapenemase-mediated resistance poses a far greater threat. Objectives: The aim of the study was to detect the underlying mechanisms of resistance of carbapenem resistant gram negative bacteria by phenotypic detection (lateral flow immunoassay) directly from positive blood cultures& genotypic detection (Conventional PCR). Methodology: This research was performed on clinical isolates of 25 positive blood cultures of carbapenem resistant Gram -ve bacteria from pediatric cases at Beni-Suef University Hospital. The isolates underwent identification & antimicrobial susceptibility testing by means of VITEK 2 compact system. We assessed a rapid lateral flow immunoassay (LFIA) directly on positive blood culture bottles for carbapenemase genes (OXA-48, KPC, NDM, VIM,  and IMP)  & compared outcomes with those obtained utilizing conventional polymerase chain reaction (PCR) techniques. Results: PCR test presented that OXA-48 was the most predominant genotype (96%), and the least prevalent was IMP (56%). We evaluated the results of the lateral flow immunoassay and found that the sensitivity of each type was in the range of 71.4%–100% and the specificity of each type was from 0%–90.9% in comparison with PCR as the gold standard. Conclusion: The lateral flow immunoassay is an effective & efficient phenotypic test for quickly identifying carbapenemase-generating isolates straight from blood culture bottles. It may also be utilized as a point-of-care test.

DOI

10.21608/ejmm.2024.298713.1271

Keywords

carbapenemases, lateral flow immunoassay, conventional PCR, OXA-48

Authors

First Name

Nesreen

Last Name

Kamel

MiddleName

M.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

nsreen_walid@yahoo.com

City

Beni-Suef

Orcid

0000-0002-7673-0697

First Name

Azza

Last Name

Gaber

MiddleName

A.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

nsreen.walidpt@gmail.com

City

Beni-Suef

Orcid

-

First Name

Mohamed

Last Name

Sebak

MiddleName

-

Affiliation

Department of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

Email

mohamed.sebak@pharm.bsu.edu.eg

City

Beni-Suef

Orcid

-

First Name

Aida

Last Name

Salem

MiddleName

M.S.

Affiliation

Department of Pediatrics, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

aida.mohamed@med.bsu.edu.eg

City

Beni-Suef

Orcid

-

First Name

Ehab

Last Name

Fahmy

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Helwan University, Helwan, Egypt

Email

ehabfahmy000@gmail.com

City

Helwan

Orcid

-

First Name

Mai

Last Name

Ahmed

MiddleName

E.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

dr.meno@hotmail.com

City

Beni-Suef

Orcid

-

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-07-04

Publish Date

2024-07-01

Page Start

165

Page End

171

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 165-171.pdf

PDF . 352.8kB

Link

https://ejmm.journals.ekb.eg/article_370903.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=370903

Order

370,903

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phenotypic And Genotypic Characterization Of Carbapenem – Resistant Gram – Negative Bacteria Among Pediatrics With Positive Blood Cultures

Details

Type

Article

Created At

28 Dec 2024