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368793

Genotyping of Staphylococcal Species on the Skin of Patients with Atopic Dermatitis

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Atopic dermatitis (AD) is a prevalent illness that impacts a diverse range of individuals worldwide. The skin microbiome of AD patients is characterized by increased colonization by Staphylococci and decreased bacterial diversity, which could worsen disease symptoms. Objectives: To recognize staphylococcal species on skin of AD patients and to assess the association of these species with disease severity. Methodology: The study comprised 45 AD patients and 45 healthy controls. Full history, general and dermatological examination were performed to all the participants. Disease severity was calculated using Severity Scoring of Atopic Dermatitis (SCORAD). Microbiological examination was conducted for all skin specimens collected. Molecular identification of the detected staphylococcal species using multiplex Polymerase Chain Reaction (PCR). Results: There was a statistically significant higher growth of Staphylococci among cases than control group (97.8% versus 46.7%, respectively, P value<0.001). Following Genotyping, a statistically significant higher growth of Staphylococcus aureus (S. aureus) and S. hominis were detected among AD cases than control group (P value =0.0005 and 0.05, respectively). There was significant association (p value < 0.001) between severity of AD and genotypic distribution of staphylococcus species. All Patients with Severe AD were colonized with S. aureus. Conclusion: Staphylococcal species were relatively exclusive to AD. Increased frequency of S. aureus and S. hominis were observed in AD skin. These findings may be significant for understanding pathophysiology and severity of AD to outline new treatment options.

DOI

10.21608/ejmm.2024.303040.1274

Keywords

atopic dermatitis, Staphylococcus aureus, Genotyping, SCORAD

Authors

First Name

Sara

Last Name

Fouad

MiddleName

H.

Affiliation

Dermatology, Andrology and STDs Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

sara_hamdy@mans.edu.eg

City

-

Orcid

-

First Name

Nouran

Last Name

Mansour

MiddleName

H.

Affiliation

Dermatology Resident, Belqas General Hospital, Egypt

Email

nouranhassan779@gmail.com

City

-

Orcid

-

First Name

Noha

Last Name

Mahmoud

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt; Medical Microbiology and Immunology Department, Faculty of Medicine, Horus University, New Damietta, Egypt

Email

nohamostafa_81@yahoo.com

City

Mansoura

Orcid

https://orcid.org/00

First Name

Maha

Last Name

El Gayyar

MiddleName

A.

Affiliation

Dermatology, Andrology and STDs Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

elgayar_m@mans.edu.eg

City

-

Orcid

-

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-07-09

Publish Date

2024-07-01

Page Start

153

Page End

163

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 153-163.pdf

PDF . 611.4kB

Link

https://ejmm.journals.ekb.eg/article_368793.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=368793

Order

368,793

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Genotyping of Staphylococcal Species on the Skin of Patients with Atopic Dermatitis

Details

Type

Article

Created At

28 Dec 2024