Background: Antimicrobial resistance is increasing, particularly in Gram-negative bacteria such as Klebsiella species, which can cause acute infections and complications in intensive care units. Objectives: This study aims to investigate antimicrobial susceptibility patterns and the frequency of resistance among clinical Klebsiella isolates, focusing on prevalence of extended spectrum β-lactamases. Methodology: One hundred and eighty-one clinical isolates of Klebsiella spp. obtained from the Kasr Al Ainy Educational Hospital's Microbiological Laboratorywere subjected to phenotypic identification and antibiotics susceptibility testing using the Vitek 2 system. Results: 115 isolates out of 181 were identified as Klebsiella species. Of which, K. pneumoniae represented 89% while K. ozaenae and K. oxytoca were 7 and 4%, respectively. Only 53 isolates displayed extended spectrum β-lactamases (ESBL) Klebsiella. Throughout the susceptibility test of thirteen antibiotics, the highest resistant pattern (100%) was observed against ampicillin. Furthermore, ESBL Klebsiella spp. exhibited high resistance against cefotaxime, amikacin, and ciprofloxacin. Conclusion: This investigation revealed that K. pneumoniae subsp. pneumoniae was the dominant followed by K. pneumoniae subsp. ozaenae, and K. oxytoca. The highest resistance was identified among ESBL-producing clinical isolates; also, our results indicate extended drug resistance among non-ESBL-producing isolates.