ManageChatDiscover
366539

Characterization of aminoglycoside-resistant Enterobacteriaceae isolated from inanimate hospital surfaces in Egypt

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: The emergence of antibiotic resistance is one of the major issues facing global healthcare systems. Objectives: This study aims to determine the prevalence and resistance profiles of Enterobacteriaceae isolated from inanimate surfaces of an Egyptian hospital. Methodology: MALDI-TOF identified Gram-negative bacteria and the antibiotic resistance profile of Enterobacteriaceae isolates was determined by Vitek 2 system. Results: From the inanimate surfaces, 266 isolates of Gram-negative nosocomial bacteria were identified of which 175 (65.79%) belonged to Enterobacteriaceae. The most frequently isolated bacteria were Klebsiella pneumoniae (n=72), followed by Escherichia coli (n=65) while Acinetobacter baumannii (n=54) and Pseudomonas aeruginosa (n=21) were the dominant non-Enterobacteriaceae isolates. Moreover, the Enterobacteriaceae isolates exhibited high degrees of resistance against aminoglycosides, penicillins, and carbapenem. In addition, various aminoglycoside-resistance genes were detected by the polymerase chain reaction (PCR). Results revealed that most Enterobacteriaceae isolates harbours aac(3′)-Ib gene (89.1%) followed by aph(3′)-Ia (52.5%) and aac(3)-II (50.2%). Conclusion: Our findings demonstrate that inanimate surfaces may be potential reservoirs of resistant Gram-negative bacteria, which directly threaten hospitalized patients.

DOI

10.21608/ejmm.2024.299848.1269

Keywords

Aminoglycoside resistance, Inanimate surface, Enterobacteriaceae prevalence, Nosocomial infection

Authors

First Name

Amr

Last Name

Fahmy

MiddleName

H.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

amrhosny820@gmail.com

City

-

Orcid

-

First Name

Khaled

Last Name

El-Dogdog

MiddleName

A.

Affiliation

Microbiology Department, Faculty of Agriculture, Ain Shams University

Email

hazemmgfarahat@gmail.com

City

-

Orcid

-

First Name

El-Sayed

Last Name

Abd-Elsalam

MiddleName

T.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

tsayed1969@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Salah

MiddleName

G.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Email

farahat@sci.cu.edu.eg

City

-

Orcid

0000-0002-0872-0024

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-06-28

Publish Date

2024-07-01

Page Start

119

Page End

124

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 119-124.pdf

PDF . 421.9kB

Link

https://ejmm.journals.ekb.eg/article_366539.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=366539

Order

366,539

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Characterization of aminoglycoside-resistant Enterobacteriaceae isolated from inanimate hospital surfaces in Egypt

Details

Type

Article

Created At

28 Dec 2024