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Phenotypic and genotypic detection of extended spectrum beta lactamase producing Escherichia coli in urinary tract infection of pregnant women in Benha

Article

Last updated: 05 Jan 2025

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Tags

Clinical microbiology

Abstract

Background: Urinary Tract Infections (UTIs) caused by extended spectrum beta lactamase (ESBL) producing E.coli in pregnancy is a serious health problem increasing pregnancy–related complications and continue to be a challenge for choosing the suitable therapy since they may exhibit coresistance to many other classes of antibiotics. Objectives:  to ascertain the genotypic and phenotypic characteristics of (ESBLs) producing E. coli from pregnant females attending the Obstetrics and Gynecology Department in Benha University hospitals. Methodology:  100 urine samples of pregnant females were presumptively identified using standard bacteriological methods. Positive cultures were tested for antibiotic sensitivity.   Production of ESBL was confirmed by double disc synergy and MALDI-TOF MS assay. Loop mediated isothermal amplification (LAMP) was further applied for the ESBL gene genotypic detection. Results:  34(82.93%) women out of 100 were colonized by ESBL producing E.coli. The rates of antibiotic susceptibility of E.coli isolates were 53.66% to Ampicillin, 43.9% to pipracillin, 30.9% to gentamicin, 43.9% to aztreonam, 21.9%, 39% and 17% to ceftriaxone, cefotaxime and cefpodoxim respectively. Concerning genotypic detection of ESBLs genes; CTX M-1 was (29.2%), followed by CTX M-2   (4.9%). Conclusion occurrence of ESBL synthesis among E. coli isolates is high. Periodical detection and checking of antimicrobial susceptibility of ESBL isolates in pregnant women, and the use of effective antimicrobial drugs are recommended to decrease the risk of high antibiotic resistance rate and reduce potential subsequent complications.

DOI

10.21608/ejmm.2024.297861.1265

Keywords

UTI, Extended-spectrum beta-lactamases, Escherichia coli, pregnant females

Authors

First Name

Nagah

Last Name

Riham

MiddleName

R.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

rnragab@yahoo.com

City

Benha

Orcid

-

First Name

Heba-Allah

Last Name

EL-Marakby

MiddleName

F.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

heba.fo2ad@yahoo.com

City

-

Orcid

-

First Name

Amira

Last Name

Khalil

MiddleName

E.

Affiliation

Obstetrics and Gynecology Department, Faculty of Medicine, Benha University

Email

-

City

-

Orcid

-

First Name

Nora

Last Name

Nassar

MiddleName

M.

Affiliation

Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

First Name

Omnia

Last Name

Habashy

MiddleName

Y.

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

Shaker

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

doaashaker2012@yahoo.com

City

-

Orcid

-

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-06-18

Publish Date

2024-07-01

Page Start

95

Page End

102

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 95-102.pdf

PDF . 500.0kB

Link

https://ejmm.journals.ekb.eg/article_363871.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=363871

Order

363,871

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phenotypic and genotypic detection of extended spectrum beta lactamase producing Escherichia coli in urinary tract infection of pregnant women in Benha

Details

Type

Article

Created At

28 Dec 2024