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363359

Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Overexpression of Acr AB and Oxq AB efflux pump genes in Klebsiella pneumoniae could increase its resistance to antimicrobials. Objectives: to explore the prevalence of Acr AB and Oxq AB efflux pump genes in resistant isolates of K. pneumoniae and to detect their role in conferring resistance to ciprofloxacin. Methodology:  Isolation of K. pneumoniae was followed by detection of its antimicrobial susceptibility. Then, the ciprofloxacin minimum inhibitory concentrations (MIC) were analyzed by broth microdilution method before and after treatment with the efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone.  The presence of Acr AB and Oxq AB efflux pump genes was investigated by polymerase chain reaction. Results: One hundred K. pneumonia strains were isolated and 65 % of these isolates were multidrug-resistant with high resistance for sulphamethoxazole (77%), amikacin (66%), and ciprofloxacin (63%). 60.3% of ciprofloxacin-resistant K. pneumoniae had a significant 4-fold decrease or more reduction in MIC for ciprofloxacin after CCCP addition (p value<0.05).  There was a significant difference concerning the frequency of efflux pump genes in ciprofloxacin-resistant isolates as 82.5% were positive and acrA, acrB, oqxA, and oqxB were detected in 77.7, 71.4, 62 and 65% of isolates, respectively. Conclusion: Acr AB and Oxq AB efflux pump genes were prevalent among K. pneumoniae isolates and they had an essential role in explaining their resistance to ciprofloxacin.

DOI

10.21608/ejmm.2024.297587.1264

Keywords

K. pneumoniae, efflux pump, and ciprofloxacin

Authors

First Name

Hend

Last Name

El-sayed

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

dr_haem85@yahoo.com

City

-

Orcid

-

First Name

Hanaa

Last Name

El Maghraby

MiddleName

M.

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

hmmaghrabi@medicine.zu.edu.eg

City

-

Orcid

-

First Name

Samia

Last Name

Hussein

MiddleName

-

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

samiahussein82@hotmail.com

City

-

Orcid

-

First Name

Doaa

Last Name

El Azawy

MiddleName

Sh.

Affiliation

Anesthesia and Intensive Care Unit Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

dsali@medicine.zu.edu.eg

City

-

Orcid

-

First Name

Osama

Last Name

Attia

MiddleName

-

Affiliation

Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

ommattia2@gmail.com

City

-

Orcid

-

First Name

Bassam

Last Name

Mousa

MiddleName

-

Affiliation

General Surgery Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

doc.bassam@live.com

City

-

Orcid

-

First Name

Eman

Last Name

Orabi

MiddleName

-

Affiliation

Public Health and Community Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

dr.eman.elshahat@gmail.com

City

-

Orcid

-

First Name

Yasmin

Last Name

Fahmy

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

yamohamd@medicine.zu.edu.eg

City

-

Orcid

-

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-06-14

Publish Date

2024-07-01

Page Start

67

Page End

75

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 67-75.pdf

PDF . 475.7kB

Link

https://ejmm.journals.ekb.eg/article_363359.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=363359

Order

363,359

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals

Details

Type

Article

Created At

28 Dec 2024