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Virulence genes and antibiograms of Enterococci isolated from Intensive Care Unit patients in National Liver Institute

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Enterococci are normal flora of human intestine causing several infections such as infective endocarditis, bloodstream infections, and intra pelvic/abdominal abscess especially among patients in ICU. Different virulent factors are produced by the bacterium to enhance their pathogenicity. Objectives: The aim of the study was to evaluate virulence genes and pattern of antibiotic susceptibility of Enterococcus spp. taken from ICU patients admitted at NLI. Methodology: From 140 ICU patients admitted at NLI, samples were taken after 48 hours from admission, and cultured on bile esculin agar, the GP-ID cards of VITEK-2 system used to confirm enterococcal isolation and species identification. Antibiotic susceptibility was done using VITEK2 AST-P592 cards. Multiplex-PCR was used for identification of gelE, asa1, esp genes. Results: esp gene was significantly high in E. faecium (p =0.004). The virulence genes combinations were significant between the enterococcal species (p = 0.004). A significant correlation was found between Enterococci isolates clinical source and esp gene (p =0.012). Antibiotic susceptibilities were variable among enterococcal isolates. The resistance to ampicillin and streptomycin (high level synergy) was significant between E. faecium and E. faecalis (p =0.014, 0.006) respectively. No association was observed regarding antibiotic susceptibility with genes of virulence (p > 0.05). Conclusion: A relationship was found between distribution pattern of virulence genes and the enterococcal species. A correlation was found between Enterococci isolates clinical source and esp gene. Antibiotic resistance was significantly different between the enterococcal species. Surveillance of drug resistance should be done regularly for proper antibiotics selection.

DOI

10.21608/ejmm.2024.296382.1261

Keywords

Virulence genes, Antibiotic Susceptibility, enterococci

Authors

First Name

Sara

Last Name

Ayoub

MiddleName

A.M.

Affiliation

Medical Microbiology & Immunology Department, National Liver Institute, Menoufia University

Email

saraayoub@liver.menofia.edu.eg

City

Shebin elkom

Orcid

-

First Name

Azza

Last Name

Abd El Aziz

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, National Liver Institute, Menoufia University

Email

azzazoze@gmail.com

City

-

Orcid

-

First Name

Tawfik

Last Name

Abd El Moteleb

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, National Liver Institute, Menoufia University

Email

tawfeekhassan815@gmail.com

City

-

Orcid

-

First Name

Minatallah

Last Name

Elshafie

MiddleName

-

Affiliation

Anaesthesia and intensive care Department, National Liver Institute - Menoufia University

Email

menna80mas@gmail.com

City

-

Orcid

-

First Name

Samah

Last Name

Awad

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, National Liver Institute, Menoufia University

Email

samahawad2016@yahoo.com

City

Menoufia

Orcid

-

Volume

33

Article Issue

3

Related Issue

47674

Issue Date

2024-07-01

Receive Date

2024-06-09

Publish Date

2024-07-01

Page Start

57

Page End

65

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 3_Pages 57-65.pdf

PDF . 503.4kB

Link

https://ejmm.journals.ekb.eg/article_361870.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=361870

Order

361,870

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Virulence genes and antibiograms of Enterococci isolated from Intensive Care Unit patients in National Liver Institute

Details

Type

Article

Created At

28 Dec 2024