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354937

Dissemination of NDM-1 and OXA-48 Co-producing Carbapenem-resistant Enterobacterales at Two Tertiary Hospitals in Egypt

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background. The global incidence of carbapenem-resistant Enterobacterales (CRE) is increasing, posing a major treatment challenge and serious infection control concern. Objective. We aimed to characterize carbapenemases both genotypically and phenotypically among CRE isolated from two tertiary hospitals in Egypt, and to detect the genetic relatedness among studied isolates. Methodology. A total of 107 non-duplicate CRE strains isolated from different clinical specimens of hospitalized patients were assessed for presence of carbapenemases genes using multiplex PCR. One-hundred isolates were also assessed for the presence of carbapenemases using MASTDISCS Combi Carba Plus kit. Molecular typing of the isolates was done by Enterobacterial Repetitive Intergenic Consensus region PCR (ERIC-PCR). Results. Klebsiella pneumoniae (K. pneumoniae) represented most of the studied isolates (n=85; 79.4%). The most prevalent carbapenemase gene among our isolates was blaNDM-1 (n=103; 96.2%) followed by blaOXA-48 (n=68; 63.5%). Among them, 64 isolates (59.8%) carried more than one gene as blaNDM-1 and blaOXA-48 genes. blaKPC, blaVIM and blaIMP were not detected in the isolates. The sensitivity of the Carba Plus kit for the detection of MBL was much better than OXA-48 (82.29% and 9.52%, respectively), however, the negative predictive value was poor for the detection of both (19.05% and 39.36%, respectively). The constructed dendrogram for each genus from each hospital revealed significant genetic diversity among the investigated isolates. Conclusions. The study highlights the wide dissemination of diverse clones of CRE isolates that co-harbor blaOXA-48 and blaNDM-1 genes among our hospitals as well as low accuracy of detection using phenotypic techniques like Carba plus kit.

DOI

10.21608/ejmm.2024.288181.1247

Keywords

Carbapenem-resistant Enterobacterales, Carbapenemases detection, Molecular Typing, ERIC-PCR

Authors

First Name

Dalia

Last Name

ElFeky

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Egypt

Email

dselfeky@cu.edu.eg

City

-

Orcid

0000-0003-3633-3977

First Name

Alaa

Last Name

Awad

MiddleName

R.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Egypt

Email

alaa.mreda1@gmail.com

City

giza

Orcid

0000-0002-4468-4446

First Name

Hagar

Last Name

Mowafy

MiddleName

L.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Egypt

Email

hagarmowafy@kasralainy.edu.eg

City

-

Orcid

0000-0003-3837-5978

First Name

Manal

Last Name

Baddour

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Alexandria University, Egypt

Email

manal.baddour@alexmed.edu.eg

City

giza

Orcid

0000 0003 4263 2412

First Name

Reham

Last Name

Hamed

MiddleName

M.R.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Egypt

Email

rehamraafat1986@yahoo.com

City

giza

Orcid

0000-0002-5526-2134

Volume

33

Article Issue

2

Related Issue

45766

Issue Date

2024-04-01

Receive Date

2024-05-09

Publish Date

2024-04-01

Page Start

163

Page End

174

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 2_Pages 163-174.pdf

PDF . 723.2kB

Link

https://ejmm.journals.ekb.eg/article_354937.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=354937

Order

354,937

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Dissemination of NDM-1 and OXA-48 Co-producing Carbapenem-resistant Enterobacterales at Two Tertiary Hospitals in Egypt

Details

Type

Article

Created At

28 Dec 2024