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338117

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Pseudomonas aeruginosa (P. aeruginosa) infections pose a significant risk to hospitalized patients due to its high prevalence and inherent resistance mechanisms. Efflux pumps, particularly the Mex family, contribute significantly to multidrug resistance (MDR) in P. aeruginosa. Objectives: To detect phenotypic and genotypic efflux pump-mediated antibiotic resistance in P. aeruginosa isolated from patients admitted to Benha University Hospitals. Methodology: This cross-sectional descriptive study was executed on 50 P. aeruginosa strains isolated from different clinical samples of patients admitted to hospitals of Benha University. The isolates underwent identification and antimicrobial susceptibility testing by means of VITEKĀ® 2 compact system. The phenotypic identification of the efflux pumps was done by the Carbonyl Cyanide M-Chlorophenyl Hydrazone test (CCCP Test), followed by identification of different genes encoding efflux pump-mediated carbapenem resistance by PCR assays. Results: from the different clinical samples, P. aeruginosa were isolated mostly from sputum samples (30%), wound swabs (24%) and urine samples (22%). P. aeruginosa isolates exhibited varying sensitivities to tested antibiotics, showing the highest sensitivity to colistin (70%). The Minimum Inhibitory Concentration (MIC) of meropenem and imipenem significantly decreased in the existence of CCCP, indicating efflux pump involvement. PCR results revealed a high prevalence of MexA (72.4%) and MexE (79.3%) genes, correlating well with phenotypic findings. Conclusion: Efflux pumps have a significant function in mediating antibiotic resistance in P. aeruginosa isolates. The correlation between phenotypic and genotypic results, particularly the prevalence of MexA and MexE genes, underscores the importance of efflux pumps in MDR in P. aeruginosa strains from this clinical setting.

DOI

10.21608/ejmm.2024.338117

Keywords

Efflux Pumps, carbapenem, Pseudomonas aeruginosa

Authors

First Name

Wafaa

Last Name

Elmosallamy

MiddleName

A.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Benha University

Email

wafaamosallamy@hotmail.com

City

Benha

Orcid

-

First Name

Hala

Last Name

Tabl

MiddleName

A.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Benha University

Email

tablhala@yahoo.com

City

-

Orcid

-

First Name

Maha

Last Name

Ghanem

MiddleName

A.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Benha University

Email

dr.mahaghanem91@gmail.com

City

-

Orcid

29102201603028

First Name

Hasnaa

Last Name

Abd Elhamid

MiddleName

S.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Benha University

Email

hasnaashawky2@gmail.com

City

Benha

Orcid

-

Volume

33

Article Issue

2

Related Issue

45766

Issue Date

2024-04-01

Receive Date

2024-01-24

Publish Date

2024-04-01

Page Start

11

Page End

18

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 2_Pages 11-18.pdf

PDF . 420.1kB

Link

https://ejmm.journals.ekb.eg/article_338117.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=338117

Order

338,117

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Details

Type

Article

Created At

28 Dec 2024