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338110

A Comparative Study between Invasive and Superficial Candida albicans Infections Regarding Biofilm Formation, ALS3 and SAP1-6 Genes Expression and Anti-Fungal Drug Susceptibility

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Candida species are usually found as a component of individual's normal microflora. Candida albicans capable of causing opportunistic infections varying from superficial infections in immunocompetent people, to life-threatening systemic infections in immunocompromised persons. A variety of pathogenicity factors contribute to colonization and pathogenesis of C. albicans during superficial and systemic infections. Objective: The aim of this work is to compare between the expression of ALS3 and SAP1-6 genes among Candida albicans isolated from superficial versus invasive candidiasis. To study the antifungal susceptibility pattern of the collected isolates. Methodology: This study was conducted on 25 isolates of C. albicans isolated from superficial candida infection and 25 isolates from invasive infections, The isolates were assessed for their capability to form biofilm, measurement of quantitative gene expression of ALS3, SAP1-6 genes by real   time   reverse transcription -polymerase chain reaction (RT-PCR), and antifungal susceptibility using the disk diffusion method. Results: There had been a high statistically significant difference between the two groups regarding biofilm formation p value < 0.001. Also, there was a high statistically significant difference in the expression of SAP 1-6 and ALS3 genes between the two studied groups (P < 0.001) with higher expression levels in the systemic candidiasis group. Resistance to antifungals was more common in systemic isolates. Conclusion: This study has found a relationship between the biofilm development and fluconazole and itraconazole resistance in C. albicans isolates. Moreover, the present study detected a correlation between the expressions of ALS3 gene and biofilm development.

DOI

10.21608/ejmm.2024.338110

Keywords

Superficial infection, Invasive infection, biofilm formation, ALS3, SAP1-6

Authors

First Name

Marwa

Last Name

Ahmed

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

marwa.aliahmed@yahoo.com

City

-

Orcid

-

First Name

Hamida

Last Name

Goher

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

hamida_m_goher@yahoo.com

City

Cairo

Orcid

-

First Name

Reham

Last Name

Dwedar

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

rehamdewdar@gmail.com

City

Cairo

Orcid

-

First Name

Yasser

Last Name

Nassar

MiddleName

-

Affiliation

Department of Critical Care Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

ysnassar@hotmail.com

City

Cairo

Orcid

-

First Name

Mona

Last Name

Nada

MiddleName

G.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

monagnada@gmail.com

City

-

Orcid

-

First Name

Maha

Last Name

Kotb

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

kotbmaha@yahoo.com

City

-

Orcid

-

Volume

33

Article Issue

2

Related Issue

45766

Issue Date

2024-04-01

Receive Date

2024-01-24

Publish Date

2024-04-01

Page Start

1

Page End

9

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 2_Pages 1-9.pdf

PDF . 509.6kB

Link

https://ejmm.journals.ekb.eg/article_338110.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=338110

Order

338,110

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

A Comparative Study between Invasive and Superficial Candida albicans Infections Regarding Biofilm Formation, ALS3 and SAP1-6 Genes Expression and Anti-Fungal Drug Susceptibility

Details

Type

Article

Created At

28 Dec 2024