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344924

Relationship between Biofilm Formation and Production of Extended-Spectrum β-lactamases and Metallo-β-lactamases among Gram-negative Bacilli

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Gram-negative bacilli are important pathogens of hospital-acquired infections as they can survive in hospital environment. They show a wide range of antibiotic resistance. Objectives: To detect biofilm, ESBL and MBL production among Gram-negative bacilli, to correlate their relation together and assess their antibiotic resistance pattern. Methodology: Two hundred isolates of Gram-negative bacilli, collected from different clinical samples of hospitalized patients at Cairo University Hospitals, were subcultured on MacConkey's agar and identified by conventional methods. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. The ability of biofilm production was assessed using microtiter plate method. Results:  Gram-negative bacilli isolates were most frequently recovered from urine samples (53%). Biofilm formation was found in 47% of the isolates. Furthermore, strong biofilm-forming category was detected in 2% of the isolates, while moderate and weak biofilm-forming categories were detected in 13% and 85% respectively. Production of ESBL and MBL among Gram-negative bacilli isolates was 39.5 % and 49% respectively. Conclusions: Biofilm formation was associated with MBL production among Gram-negative isolates. Moreover, ESBL and MBL producers were more resistant to antibiotics than non ESBL and non MBL producers.

DOI

10.21608/ejmm.2024.344924

Keywords

ESBL, MBL, Antibiotic resistance, Biofilm, Gram-Negative

Authors

First Name

Nermin

Last Name

Rashwan

MiddleName

S.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University

Email

naramino_2005@kasralainy.edu.eg

City

Cairo

Orcid

0000- 0002- 1264- 0430

First Name

Nadia

Last Name

Ouda

MiddleName

H.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University

Email

nadiahafez@kasralainy.edu.eg

City

Cairo

Orcid

-

First Name

Maha

Last Name

Elnaggar

MiddleName

B.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University

Email

mahaelnaggar@yahoo.com

City

Cairo

Orcid

-

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2024-03-08

Publish Date

2024-01-01

Page Start

153

Page End

163

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 153-163.pdf

PDF . 647.5kB

Link

https://ejmm.journals.ekb.eg/article_344924.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=344924

Order

344,924

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Relationship between Biofilm Formation and Production of Extended-Spectrum β-lactamases and Metallo-β-lactamases among Gram-negative Bacilli

Details

Type

Article

Created At

28 Dec 2024