Beta
ManageSearchChatDiscover
328759

Impact of TLR9 gene Polymorphism on Severity of Helicobacter pylori Infection and Its Association with Virulence genes

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Helicobacter pylori colonizes stomach epithelium in the majority of people worldwide. It disturbs the local mucosa's homeostasis in the stomach and causes many pathological disorders, such as chronic gastritis, peptic ulcers and gastric cancer. Objective: This study aimed to determine the relation between different TLR9 gene polymorphism genotypes and the risk and severity of H. pylori‑related gastric diseases and determine possible associations of these genotypes with H. pylori virulence genes. Methodology: 136 adult dyspeptic patients who had gastric symptoms attended Gastroenterology Department at Suez Canal University Hospitals were incorporated in this study. Three stomach biopsies were obtained from each patient. The glmM gene was amplified using PCR for confirmation of H. pylori infection. Virulence genes were identified by PCR. Using PCR-RFLP, the genotypes of TLR9 gene polymorphism were analyzed. Results: The most prevalent genes were hsp60, vacA s1/ s2 and vacA m1/m2 detected in 93.7 % of H. pylori isolates, followed by cagA (54.2%). Frequencies of CC, TC, and TT genotypes were 41.7 %, 38.5 %, and 19.8 %, respectively in infected patients compared to 10.4 %, 54.2 %, and 35.4 %, respectively in the control group. Conclusion: This study reported a significant association between TLR9 SNP and H. pylori‑related diseases and showed that the patients who had the CC genotype may have higher risk of developing severe gastritis. There may be a synergistic effect between the CC genotype and H. pylori carrying hsp60 and vacA s1/m1 genes leading to severe gastritis.

DOI

10.21608/ejmm.2024.328759

Keywords

H. pylori, PCR, TLR9 gene polymorphism, Virulence genes

Authors

First Name

Yara

Last Name

Marei

MiddleName

E.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

yarayara253@hotmail.com

City

-

Orcid

-

First Name

Bassam

Last Name

Mansour

MiddleName

-

Affiliation

Infection and endemic disease Department, Faculty of medicine, Suez Canal University, Ismailia, Egypt

Email

bassammansour99@yahoo.com

City

-

Orcid

-

First Name

Samar

Last Name

Ahmed

MiddleName

A.

Affiliation

Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

sammoura586@gmail.com

City

-

Orcid

-

First Name

Hassnaa

Last Name

Nassar

MiddleName

M.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

hassnaa_1986@yahoo.com

City

-

Orcid

-

First Name

Eman

Last Name

Nasr El Dien

MiddleName

F.

Affiliation

Internal Medicine Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

emy_nasr55@yahoo.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Hamady

MiddleName

B.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

asmaabakeir@yahoo.com

City

Ismailia

Orcid

-

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2023-12-04

Publish Date

2024-01-01

Page Start

99

Page End

110

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 99-110.pdf

PDF . 689.8kB

Link

https://ejmm.journals.ekb.eg/article_328759.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=328759

Order

328,759

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Impact of TLR9 gene Polymorphism on Severity of Helicobacter pylori Infection and Its Association with Virulence genes

Details

Type

Article

Created At

28 Dec 2024