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327751

Antigen Detection and PCR versus Conventional Culture for Diagnosis of Campylobacter Infections in Pediatric Gastroenteritis

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Diagnostics

Abstract

Background: Campylobacter is one of the leading pathogens which causes bacterial gastroenteritis among children worldwide, especially in developing countries.  Several laboratory methods have been used to diagnose campylobacteriosis including culture, ELISA, and PCR. Objectives: The aim of this study was to compare PCR and antigen detection by ELISA with culture for the detection of Campylobacter. Methodology: The present study was conducted on 160 stool samples that were collected from pediatric patients complaining of acute gastroenteritis. All samples were cultured on Modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) and suspected colonies were consequently identified. Detection of Campylobacter antigen (PEB1) in stools was done by ELISA. Molecular detection of virulence genes: cadF, hipO, and asp in stools was done by multiplex PCR. Results: Thirty-five samples (21.9%) were found to be positive for Campylobacter by culture. Campylobacter antigen was detected in 50 samples (31.3%) by ELISA. cadF gene was detected in 47 samples (29.4%)  by PCR, 39 of which were positive for hipO gene and thus identified as Campylobacter jejuni, while asp gene was not detected in any sample. Conclusion: Alternative diagnostic tests for campylobacteriosis that do not rely on culture have become increasingly important. Nucleic acid-based techniques can detect the presence of Campylobacter infection and even distinguish between different Campylobacter species.

DOI

10.21608/ejmm.2024.327751

Keywords

Campylobacteriosis, ELISA, and PCR

Authors

First Name

Noha

Last Name

Gohar

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

nohagohar@kasralainy.edu.eg

City

cairo

Orcid

-

First Name

Nermin

Last Name

Mostafa

MiddleName

Z.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

nermin.zaky@gmail.com

City

-

Orcid

-

First Name

Azza

Last Name

Badr

MiddleName

E.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

drazzabadr@yahoo.com

City

Cairo

Orcid

-

First Name

Sara

Last Name

Tarek

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

sara.tarek2310@outlook.com

City

-

Orcid

0000-0002-5132-4019

First Name

Yasmine

Last Name

Elkholy

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

yasminelkholy@kasralainy.edu.eg

City

-

Orcid

0000-0002-0654-3415

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2023-11-27

Publish Date

2024-01-01

Page Start

83

Page End

90

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 83-90.pdf

PDF . 395.0kB

Link

https://ejmm.journals.ekb.eg/article_327751.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=327751

Order

327,751

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Antigen Detection and PCR versus Conventional Culture for Diagnosis of Campylobacter Infections in Pediatric Gastroenteritis

Details

Type

Article

Created At

28 Dec 2024