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327018

The Role of Interleukin-17 and Toll-Like Receptor 4 Gene Polymorphisms in Patients with Hepatitis C Virus and Hepatocellular Carcinoma

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Hepatocellular carcinoma (HCC) is a global health problem. HCC is the fourth common malignancy in Egypt. It is a crucial medical need to predict hepatocellular carcinoma development in cirrhotic liver patients. Objectives: this study aims to investigate the association between TLR4 gene polymorphism (rs2149356) and the probability of HCC development among chronic hepatitis C virus infected patients and clarify the role of serum IL-17 in patients with end stage liver disease and HCC in HCV infected patients. Methodology: the study included 25 patients with chronic hepatitis C infection, 25 patients with chronic hepatitis C infection and HCC, and 25 apparently healthy control subjects as controls. All participants undergone full clinical and laboratory assessment. TLR4 (rs2149356) G/T polymorphism genotyping by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and serum IL-17 detection by ELISA (enzyme-linked immunosorbent assay). Results: TLR4 (rs2149356) GG genotype was significantly associated with chronic HCV with HCC patients (72%) and chronic HCV patients (48%), while GT genotype was associated with healthy controls (48%) (P-value <0.001). Serum IL-17 was significantly elevated in chronic HCV with HCC patients than chronic HCV and control subjects (P-value <0.001). Serum IL-17 was an excellent predictor for HCC development at a cut-off value of 128.1 pg/dL with 96% sensitivity and 82% specificity. Conclusion: TLR4 (rs2149356) polymorphism and IL-17 have an important role in immunopathogenesis of HCC in chronic HCV infected patients.

DOI

10.21608/ejmm.2024.327018

Keywords

Hepatocellular carcinoma, Hepatitis C virus, TLR4, IL-17

Authors

First Name

Doaa

Last Name

Shaker

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

doaashaker2012@yahoo.com

City

Benha

Orcid

-

First Name

Ahmed

Last Name

Elgazar

MiddleName

S.

Affiliation

Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

dr_ahmedelgazar13@yahoo.com

City

Benha

Orcid

-

First Name

Rana

Last Name

Khashaba

MiddleName

A.

Affiliation

Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt; Faculty of Medicine, King Salman International University (KSIU), El Tur Branch, Egypt

Email

ranaatef2207.ra@gmail.com

City

Benha

Orcid

-

First Name

Ola

Last Name

El-Shimi

MiddleName

S.

Affiliation

Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

ola.samer@fmed.bu.edu.eg

City

-

Orcid

0000-0001-8276-8352

First Name

Shaymaa

Last Name

Abdelrahman

MiddleName

M.

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

shaimaa.bghdadi@fmed.bu.edu.eg

City

Benha

Orcid

-

First Name

Walaa

Last Name

Ibrahim

MiddleName

M.

Affiliation

Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

gendia.walaa@yahoo.com

City

Benha

Orcid

-

First Name

Riham

Last Name

Ragab

MiddleName

N.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

rnragab@yahoo.com

City

-

Orcid

-

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2023-11-22

Publish Date

2024-01-01

Page Start

57

Page End

63

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 57-63.pdf

PDF . 384.2kB

Link

https://ejmm.journals.ekb.eg/article_327018.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=327018

Order

327,018

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The Role of Interleukin-17 and Toll-Like Receptor 4 Gene Polymorphisms in Patients with Hepatitis C Virus and Hepatocellular Carcinoma

Details

Type

Article

Created At

28 Dec 2024