Beta
ManageSearchChatDiscover
325935

Comparison of two invitro phenotypic methods (Tissue Culture plate and Congo Red Agar) for Detection of Biofilm Formation by Enterococci

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background, Enterococci are Gram-positive bacteria that cause serious nosocomial infections. Bacteria tend to live in a community-like assembly called biofilm. Biofilm production aids bacterial virulence through numerous pathogenic mechanisms. Objective, our aim was to Assess the ability of Enterococci to form a biofilm and to compare two in vitro phenotypic methods for detection of Enterococcal biofilm formation. Methodology, we used 50 identified enterococcal isolates from clinical specimens to detect biofilm formation by tissue culture plate method (TCP) and Congo red agar method (CRA) then we compared the results of both methods. Results, we found that 64% of the isolates were able to form biofilm by TCP method while 38% were positive by CRA method. Congo red agar failed to detect 50% of the positive isolates detected by tissue culture plate method with a sensitivity (40.62%) and a specificity (66.67%). Conclusion, we concluded from our study that TCP is the most reliable method to detect biofilm forming bacteria when compared with CRA method.

DOI

10.21608/ejmm.2024.325935

Keywords

Tissue culture plate, Congo red agar, biofilm formation, enterococci

Authors

First Name

Mera

Last Name

Anan

MiddleName

M.G

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

mera_anan@yahoo.com

City

Egypt

Orcid

-

First Name

Abeer

Last Name

Abu-El-Azayem

MiddleName

K.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

abeer.aboualazaim@kasralainy.edu.eg

City

Egypt

Orcid

-

First Name

Sabrin

Last Name

Elkashef

MiddleName

M.M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

dr_sabrina_609@yahoo.com

City

-

Orcid

0000-0002-0661-878X

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2023-11-13

Publish Date

2024-01-01

Page Start

19

Page End

23

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 19-23.pdf

PDF . 279.0kB

Link

https://ejmm.journals.ekb.eg/article_325935.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=325935

Order

325,935

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Comparison of two invitro phenotypic methods (Tissue Culture plate and Congo Red Agar) for Detection of Biofilm Formation by Enterococci

Details

Type

Article

Created At

28 Dec 2024