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322729

Rapid Detection of Pseudomonas aeruginosa Causing Ventilator Associated Pneumonia Using Multiple Cross Displacement Amplification Technique in Intensive Care Units in Tanta Unive

Article

Last updated: 05 Jan 2025

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-

Tags

Diagnostics

Abstract

Background: Ventilator-associated pneumonia (VAP) stands as one of the critical distressing hospital infections. Pseudomonas aeruginosa (P. aeruginosa) represents a potentially fatal VAP causative agent that is contracted predominantly from intensive care units (ICUs). Prompt identification of this pathogen in the context of clinical specimens of VAP patients may open the gate for therapeutic optimization plans in that way stewarding antibiotic usage thus enhancing clinical outcomes as pathogen identification delay elicits forced empiric usage of less valuable or potentially toxic antimicrobials. Objectives: This study aimed to reveal the P. aeruginosa infection prevalence among VAP patients admitted to Tanta University Hospitals' ICUs via both multiple cross displacement amplification (MCDA) assay and phenotypic approach and to assess the sensitivity along with specificity of MCDA assay as a speedy alternative to the routine culture in favor of detection of P. aeruginosa. Methodology: Sixty respiratory specimens comprised endotracheal aspirate (ETA) in addition to broncho-alveolar lavage (BAL) fluid were retrieved from clinically diagnosed VAP patients. Specimens were investigated for the microbial content. Bacterial isolation and identification by phenotypic methods and detection of P. aeruginosa by MCDA assay were done. Results: Out of 60 samples, 50 samples showed either mono or polymicrobial growth. P. aeruginosa was the 2nd most frequently isolated microorganism after Klebsiella pneumoniae (K. pneumoniae). The MCDA assay exhibited 100% specificity, sensitivity, and accuracy in detecting P. aeruginosa. Conclusions: Instead of bacterial culture, the MCDA assay serves as straightforward, easy, quick, and practical technique aiming to "on-site" or point-of-care testing for the presence of P. aeruginosa.

DOI

10.21608/ejmm.2023.322729

Authors

First Name

Shaimaa

Last Name

Marey

MiddleName

S.E.

Affiliation

Medical Microbiology & Immunology, Faculty of Medicine, Tanta University, Egypt

Email

shimaa.marey@med.tanta.edu.eg

City

-

Orcid

-

First Name

Abd Alreheem

Last Name

Ads

MiddleName

G.

Affiliation

Medical Microbiology & Immunology, Faculty of Medicine, Tanta University, Egypt

Email

abdelreheam.adss1@med.tanta.edu.eg

City

-

Orcid

-

First Name

Azza

Last Name

Hassan

MiddleName

M.

Affiliation

Medical Microbiology & Immunology, Faculty of Medicine, Tanta University, Egypt

Email

azza.hassan@med.tanta.edu.eg

City

-

Orcid

-

First Name

Ghada

Last Name

Elbaradey

MiddleName

F.

Affiliation

Anesthesia, Surgical Intensive Care and Pain Management, Faculty of Medicine, Tanta University, Egypt

Email

ghada.elbaradi@med.tanta.edu.eg

City

-

Orcid

-

First Name

Radwa

Last Name

Eissa

MiddleName

A.

Affiliation

Medical Microbiology & Immunology, Faculty of Medicine, Tanta University, Egypt

Email

radwa.eissa@med.tanta.edu.eg

City

-

Orcid

-

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-10-23

Publish Date

2023-10-01

Page Start

103

Page End

110

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 103-110.pdf

PDF . 520.2kB

Link

https://ejmm.journals.ekb.eg/article_322729.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=322729

Order

322,729

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Rapid Detection of Pseudomonas aeruginosa Causing Ventilator Associated Pneumonia Using Multiple Cross Displacement Amplification Technique in Intensive Care Units in Tanta Unive

Details

Type

Article

Created At

28 Dec 2024