Beta
ManageSearchChatDiscover
322728

Phenotypic and Genotypic Characterization of AmpC Beta Lactamases in Acinetobacter Species Isolated from Cairo University Hospitals

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: In Egypt, the prevalence of plasmid-mediated class C (AmpC) beta-lactamases (β-lactamases) in Acinetobacter species is on the rise, and its resistance to a wide range of β-lactam drugs makes it a hazardous condition. Objectives: The aim of this research was to assess the occurrence of AmpC among Acinetobacter clinical isolates by phenotypic confirmatory tests and to compare them with PCR as the gold standard method. Methodology: The current study incorporated 50 Acinetobacter clinical isolates. Detection of AmpC-producing Acinetobacter isolates was done by two phenotypic methods; AmpC disk test with Tris-EDTA and disk potentiation test (DPT) with aminophenyl boronic acid (APB) and then compared with genotypic detection of plasmid-mediated AmpC (pAmpC) gene(s) families by multiplex PCR as the gold reference technique. Results: According to the study's findings, the frequency of AmpC-producing isolates was 68.8% by AmpC disk test, 72.9% by DPT with APB test, and 79.2% by PCR. The sensitivity of AmpC disk test and DPT with APB test was 86.8% and 92.1% respectively and the specificity was 100% for both tests. The CIT family of AmpC genes was the most common type to be found in the study (33.3%). Conclusion: Phenotypic detection of AmpC was more sensitive by DPT with APB test than the AmpC disk test with Tris-EDTA. Phenotypic methods are influenced by false-negative results. Therefore, PCR remains the gold standard for detecting different forms of pAmpC gene families and determining their precise prevalence, enabling the taking of suitable actions to restrict the spread of resistant microorganisms.

DOI

10.21608/ejmm.2023.322728

Keywords

AmpC β-lactamase, AmpC disk test, Disk potentiation test, Multiplex PCR

Authors

First Name

Dina

Last Name

Elsayed

MiddleName

M.A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Helwan University, Ain Helwan, Cairo 11795, Egypt

Email

dina.azim@med.helwan.edu.eg

City

cairo

Orcid

0009-0006-6026-7219

First Name

Eman

Last Name

El-Seidi

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt; Basic Medical Sciences Department, Faculty of Medicine, Galala University, Suez, Egypt

Email

emanelseidi@kasralainy.edu.eg

City

Cairo

Orcid

-

First Name

Laila

Last Name

Rashed

MiddleName

A.

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

lailaahmedrashed@gmail.com

City

Cairo

Orcid

-

First Name

Maha

Last Name

Kotb

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

kotbmaha@yahoo.com

City

-

Orcid

-

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-10-23

Publish Date

2023-10-01

Page Start

95

Page End

102

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 95-102.pdf

PDF . 508.6kB

Link

https://ejmm.journals.ekb.eg/article_322728.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=322728

Order

322,728

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phenotypic and Genotypic Characterization of AmpC Beta Lactamases in Acinetobacter Species Isolated from Cairo University Hospitals

Details

Type

Article

Created At

28 Dec 2024