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320674

(1-3)-β-D-Glucan Antigen Detection and PCR for Diagnosis of Invasive Fungal Lung Infections

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Invasive fungal lung infection (IFLI) is a collective term used to describe a range of opportunistic infections, caused by one or more endemic or opportunistic fungi, the most isolated microbes in these infections are Candida species, Aspergillus species and Cryptococcus species. Clinical symptoms typically manifest as fever, cough, dyspnea, chest pain, and hemoptysis, Radiologic examination showed various presentations, few had typical features, such as denseness, cavitation, a halo sign some even showed negative results. For diagnosis; microscopy, fungal culture, galactomannan antigen, and PCR are useful tests. Other tools include imaging facilities and blood serum biomarkers. Antigen detection methods include (1-3)-β-D-Glucan lacks higher sensitivity, PCR hasn't been standardized, in this study, we evaluated serum (1-3)-β-D-Glucan antigen in comparison with PCR for diagnosis accuracy. Objective: This study aimed to evaluate serum (1-3)-β-D-Glucan antigen detection and PCR methods for diagnosis of invasive fungal lung infections. Methodology: The study conducted a cross-sectional comparative study on suspected IFLI patients and a healthy control group. Clinical and radiological criteria were used for patient selection. Blood and bronchoalveolar lavage (BAL) samples were collected for biomarker assessment. PCR and BDG antigen detection were evaluated. Results: Male patients accounted for 78.1%, with no significant gender difference. Hypertension (40%), followed by diabetes (22%), were identified as the most common risk factors. BDG antigen levels were significantly associated with fungal infections. Sensitivity was 92%, specificity 80%, with an AUC of 0.879. BDG levels correlated with treatment outcomes. Conclusion: Elevated BDG levels were associated with cancer and COPD patients. While BDG provides supportive evidence for fungal infection, a multifaceted approach is crucial. Implementing new diagnostic tools and centralizing laboratories of fungal identification in clinical setting are essential as the diagnostic landscape evolves.

DOI

10.21608/ejmm.2023.320674

Keywords

(1-3)-β-D-Glucan Ag, PCR, Invasive, FUNGAL, lung, culture

Authors

First Name

Wafaa

Last Name

Elkasaby

MiddleName

S.

Affiliation

Resident of Medical Microbiology - New General Mansoura Hospital - Ministry of Health

Email

dr.wafaa.sadek@gmail.com

City

-

Orcid

-

First Name

Fikry

Last Name

El-Morsy

MiddleName

E.

Affiliation

Department of Medical Microbiology and Immunology Faculty of Medicine, Mansoura University

Email

-

City

Mansoura

Orcid

-

First Name

Mohammad

Last Name

El badrawy

MiddleName

K.F.

Affiliation

Professor of Chest medicine Faculty of Medicine, Mansoura University

Email

-

City

Mansoura

Orcid

-

First Name

Niveen

Last Name

El-wakeel

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology Faculty of Medicine, Mansoura University

Email

-

City

-

Orcid

-

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-10-09

Publish Date

2023-10-01

Page Start

71

Page End

76

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 71-76.pdf

PDF . 355.7kB

Link

https://ejmm.journals.ekb.eg/article_320674.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=320674

Order

320,674

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

(1-3)-β-D-Glucan Antigen Detection and PCR for Diagnosis of Invasive Fungal Lung Infections

Details

Type

Article

Created At

28 Dec 2024