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317383

Speciation and Antifungal Susceptibility of Clinical Non-albicans Candida isolated from Patients in a Tertiary Care Centre in Egypt

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: There has been a significant rise in morbidity and mortality due to infections caused by Candida spp. with an upsurge in the incidence of non-albicans Candida (NAC), where the clinical outcome is affected by their decreased susceptibility to azoles. Accurate identification is an important step that leads to selecting a suitable antifungal agent. Objectives: This study aimed to identify the main NAC clinical spp. in the hospital setting and to determine the antifungal susceptibility. Methodology: 133 isolates of Candida were identified by culture on Sabouraud Dextrose Agar, Gram stain, and germ tube test. Isolates were then subcultured on HiCrome chromogenic agar medium, and identification was confirmed using Vitek 2 automated compact system. NAC isolates antifungal susceptibility was detected using disk diffusion method and Vitek 2 compact system. Results: The majority of the Candida isolates (75%) were identified as NAC spp. with C. glabrata predominance (96%). HiCrome showed a sensitivity of 97% compared to Vitek 2 system. Disk diffusion showed high sensitivity when tested with amphotericin B, fluconazole, voriconazole and caspofungin of 100%, 95.2%, 100%, and 92.86% respectively compared to Vitek 2. Conclusion: This study confirms the substantial shift in Candida spp. from albicans to NAC. HiCrome chromogenic agar medium could be used as a rapid method for identification of the common NAC spp. Disk diffusion represents a reliable alternative method for routine antifungal susceptibly testing in the absence of automated systems.

DOI

10.21608/ejmm.2023.317383

Keywords

Non-albicans Candida (NAC), HiCrome chromogenic media, Antifungal susceptibility, VITEK 2

Authors

First Name

Heba

Last Name

Dahroug

MiddleName

M.

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

hebba.dahroug.hd@gmail.com

City

-

Orcid

-

First Name

Dalia

Last Name

Salem

MiddleName

A.

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

drdaliasalem@gmail.com

City

-

Orcid

-

First Name

Basma

Last Name

Tarek

MiddleName

-

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

basma.tarek.456@gmail.com

City

-

Orcid

-

First Name

Doaa

Last Name

Gamal

MiddleName

-

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

doaagamal@gmail.com

City

-

Orcid

-

First Name

Hoda

Last Name

Helmy

MiddleName

-

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

hodahelmi@hotmail.com

City

-

Orcid

-

First Name

Inas

Last Name

El-Defrawy

MiddleName

-

Affiliation

Microbiology Department, Theodor Bilharz Research Institute (TBRI), Egypt

Email

inasdef@yahoo.com

City

-

Orcid

-

First Name

Mohammed

Last Name

Ismail

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

amrkholy@hotmail.com

City

Cairo

Orcid

-

First Name

Rania

Last Name

Shash

MiddleName

Y.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

raniayahiashash@cu.edu.eg

City

-

Orcid

0000-0002-6039-3737

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-09-14

Publish Date

2023-10-01

Page Start

23

Page End

31

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 23-31.pdf

PDF . 550.1kB

Link

https://ejmm.journals.ekb.eg/article_317383.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=317383

Order

317,383

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Speciation and Antifungal Susceptibility of Clinical Non-albicans Candida isolated from Patients in a Tertiary Care Centre in Egypt

Details

Type

Article

Created At

28 Dec 2024